Cui Lianmin, Zhang Ling, Zeng Heping
School of Optical-Electrical and Computer Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China.
Public Experiment Center, University of Shanghai for Science and Technology, Shanghai 200093, China.
Nanomaterials (Basel). 2021 Nov 1;11(11):2927. doi: 10.3390/nano11112927.
Fluorescence resonance energy transfers (FRET) between cyan fluorescent protein (CFP) and yellow fluorescent protein (YFP) on nanoporous gold (NPG) are systematically investigated by controlling the distance between NPG and fluorescent proteins with polyelectrolyte multilayers. The FRET between CFP and YFP is significantly enhanced by NPG, and the maximum enhancement is related to both ligament size of NPG and the distance between NPG and proteins. With the optimized distance, 18-fold FRET enhancement was obtained on NPG compared to that on glass, and the conversion efficiency is about 90%. The potential to tune the characteristic energy transfer distance has implications for applications in nanophotonic devices and provides a possible way to design sensors and light energy converters.
通过聚电解质多层膜控制纳米多孔金（NPG）与荧光蛋白之间的距离，系统地研究了纳米多孔金上的青色荧光蛋白（CFP）与黄色荧光蛋白（YFP）之间的荧光共振能量转移（FRET）。NPG显著增强了CFP与YFP之间的FRET，最大增强与NPG的韧带尺寸以及NPG与蛋白之间的距离有关。在优化的距离下，与玻璃相比，NPG上的FRET增强了18倍，转换效率约为90%。调节特征能量转移距离的潜力对纳米光子器件的应用具有重要意义，并为设计传感器和光能转换器提供了一种可能的方法。
