College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, 430072, PR China.
College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, 430072, PR China; Animal, Plant and Foodstuffs Inspection Center of Tianjin Customs, Tianjin, 300461, PR China.
Biosens Bioelectron. 2022 Feb 15;198:113819. doi: 10.1016/j.bios.2021.113819. Epub 2021 Nov 19.
Personal glucose meter (PGM) is one of the most commercially available POC (point-of-care) devices for monitoring the level of glucose reliably, yet its non-glucose quantification ability is limited since such strategy needs ingenious interface design and tedious enzyme conjugation. Herein, we constructed a portable and sensitive platform that can detect non-glucose target by combining enzyme-encapsulated zeolitic imidazole framework-90 (ZIF-90) with personal glucose meter. ZIF-90 is an ideal carrier and susceptor due to the extraordinary capability of packaging enzyme and stimuli-responsiveness. We selected adenosine-5'-triphosphate (ATP) as the target model of non-glucose analytes. Upon ATP-induced decomposition of MOF, the released enzyme (glucose oxidase or invertase) catalyzed substrate and gave rise to the change of the glucose concentration for PGM assay. This method determined ATP with a remarkably sensitivity of 233 nM and effective recovery in real serum samples. Our strategy provides a facile and practical approach for measuring the non-glucose target using PGM, and could potentially be applied in bimolecular detection in point-of-care diagnosis.
个人血糖仪（PGM）是最商业化的即时检测（POC）设备之一，可用于可靠地监测葡萄糖水平，但由于这种策略需要巧妙的界面设计和繁琐的酶偶联，其非葡萄糖定量能力有限。在此，我们构建了一个便携式和灵敏的平台，可通过将包封酶的沸石咪唑骨架-90（ZIF-90）与个人血糖仪相结合来检测非葡萄糖靶标。ZIF-90 是一种理想的载体和敏感器，因为它具有包装酶和刺激响应的非凡能力。我们选择腺嘌呤核苷三磷酸（ATP）作为非葡萄糖分析物的靶标模型。在 MOF 被 ATP 诱导分解后，释放的酶（葡萄糖氧化酶或转化酶）催化底物，并导致 PGM 分析中葡萄糖浓度的变化。该方法对 ATP 的检测灵敏度非常高，为 233 nM，并且在实际血清样本中具有有效的回收率。我们的策略为使用 PGM 测量非葡萄糖靶标提供了一种简单实用的方法，并且有可能应用于即时诊断中的双分子检测。
