Lin Bingqian, Liu Dan, Yan Jinmao, Qiao Zhi, Zhong Yunxin, Yan Jiawei, Zhu Zhi, Ji Tianhai, Yang Chaoyong James
State Key Laboratory of Physical Chemistry of Solid Surfaces, The MOE Key Laboratory of Spectrochemical Analysis & Instrumentation, Collaborative Innovation Center of Chemistry for Energy Materials, Key Laboratory for Chemical Biology of Fujian Province, and Department of Chemical Biology, College of Chemistry and Chemical Engineering, Affiliated Chenggong Hospital, Xiamen University , Xiamen 361005, People's Republic of China.
ACS Appl Mater Interfaces. 2016 Mar 23;8(11):6890-7. doi: 10.1021/acsami.6b00777. Epub 2016 Mar 8.
There is considerable demand for sensitive, selective, and portable detection of disease-associated proteins, particularly in clinical practice and diagnostic applications. Portable devices are highly desired for detection of disease biomarkers in daily life due to the advantages of being simple, rapid, user-friendly, and low-cost. Herein we report an enzyme-encapsulated liposome-linked immunosorbent assay for sensitive detection of proteins using personal glucose meters (PGM) for portable quantitative readout. Liposomes encapsulating a large amount of amyloglucosidase or invertase are surface-coated with recognition elements such as aptamers or antibodies for target recognition. By translating molecular recognition signal into a large amount of glucose with the encapsulated enzyme, disease biomarkers such as thrombin or C-reactive protein (CRP) can be quantitatively detected by a PGM with a high detection limit of 1.8 or 0.30 nM, respectively. With the advantages of portability, ease of use, and low-cost, the method reported here has potential for portable and quantitative detection of various targets for different POC testing scenarios, such as rapid diagnosis in clinic offices, health monitoring at the bedside, and chemical/biochemical safety control in the field.
对于疾病相关蛋白质的灵敏、特异且便携的检测有着相当大的需求，尤其是在临床实践和诊断应用中。由于具有简单、快速、用户友好和低成本的优点，便携式设备在日常生活中检测疾病生物标志物方面备受青睐。在此，我们报道了一种酶包封脂质体连接免疫吸附测定法，用于使用个人血糖仪（PGM）进行蛋白质的灵敏检测，以实现便携式定量读数。包封大量淀粉葡萄糖苷酶或转化酶的脂质体表面涂覆有适体或抗体等识别元件用于靶标识别。通过用包封的酶将分子识别信号转化为大量葡萄糖，凝血酶或C反应蛋白（CRP）等疾病生物标志物可分别通过PGM进行定量检测，检测限分别高达1.8或0.30 nM。凭借便携、易用和低成本的优点，本文报道的方法具有在不同的即时检测场景中对各种靶标进行便携式定量检测的潜力，例如诊所办公室的快速诊断、床边健康监测以及现场化学/生化安全控制。
