通过磷-31 磁共振波谱指纹分析（P-MRSF）快速体内定量肌酸激酶活性。

Rapid In Vivo Quantification of Creatine Kinase Activity by Phosphorous-31 Magnetic Resonance Spectroscopic Fingerprinting (P-MRSF).

机构信息

Department of Biomedical Engineering, Case Western Reserve University, Cleveland, OH, USA.

出版信息

Methods Mol Biol. 2022;2393:597-609. doi: 10.1007/978-1-0716-1803-5_31.

Abstract

Creatine kinase (CK) plays an important role in tissue metabolism by providing a buffering mechanism for maintaining a constant supply of adenosine triphosphate (ATP) during metabolic perturbations. Phosphorous-31 magnetic resonance spectroscopy (P-MRS) employing magnetization transfer techniques is the only noninvasive method for measuring the rate of ATP synthesis via creatine kinase. However, due to the low concentrations of phosphate metabolites, current P-MRS methods require long acquisition time to achieve adequate measurement accuracy. In this chapter, we present a new framework of data acquisition and parameter estimation, the P magnetic resonance spectroscopic fingerprinting (P-MRSF) method, for rapid quantification of CK reaction rate constant in the hindlimb of small laboratory animals.

摘要

肌酸激酶（CK）在组织代谢中起着重要作用，它为代谢扰动期间维持三磷酸腺苷（ATP）的恒定供应提供了缓冲机制。采用磁化转移技术的磷-31 磁共振波谱（P-MRS）是唯一的非侵入性方法，可通过肌酸激酶测量 ATP 合成的速率。然而，由于磷酸盐代谢物的浓度较低，目前的 P-MRS 方法需要较长的采集时间才能达到足够的测量精度。在本章中，我们提出了一种新的数据采集和参数估计框架，即磷磁共振波谱指纹图谱（P-MRSF）方法，用于快速定量小动物后肢中的 CK 反应速率常数。