Sir Peter Mansfield Imaging Centre, University of Nottingham, Nottingham, Nottinghamshire, United Kingdom.
Magn Reson Med. 2018 Jan;79(1):22-30. doi: 10.1002/mrm.26663. Epub 2017 Mar 16.
In vivo P magnetic resonance spectroscopy (MRS) magnetization transfer (MT) provides a direct measure of neuronal activity at the metabolic level. This work aims to use functional P MRS-MT to investigate the change in cerebral adenosine triphosphate (ATP) metabolic rates in healthy adults upon repeated visual stimuli.
A magnetization saturation transfer sequence with narrowband selective saturation of γ-ATP was developed for P MT experiments at 3 T.
Using progressive saturation of γ-ATP, the intrinsic T relaxation times of phosphocreatine (PCr) and inorganic phosphate (Pi) at 3 T were measured to be 5.1 ± 0.8 s and 3.0 ± 1.4 s, respectively. Using steady-state saturation of γ-ATP, a significant 24% ± 14% and 11% ± 7% increase in the forward creatine kinase (CK) pseudo-first-order reaction rate constant, k , was observed upon visual stimulation in the first and second cycles, respectively, of a paradigm consisting of 10-minute rest followed by 10-minute stimulation, with the measured baseline k being 0.35 ± 0.04 s . No significant changes in forward ATP synthase reaction rate, PCr/γ-ATP, Pi/γ-ATP, and nicotinamide adenine dinucleotide/γ-ATP ratios, or intracellular pH were detected upon stimulation.
This work demonstrates the potential of studying cerebral bioenergetics using functional P MRS-MT to determine the change in the forward CK reaction rate at 3 T. Magn Reson Med 79:22-30, 2018. © 2017 The Authors Magnetic Resonance in Medicine published by Wiley Periodicals, Inc. on behalf of International Society for Magnetic Resonance in Medicine. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
体内质子磁共振波谱（MRS）磁化转移（MT）可在代谢水平上直接测量神经元的活性。本研究旨在使用功能质子 MRS-MT 来研究健康成年人在重复视觉刺激下大脑三磷酸腺苷（ATP）代谢率的变化。
开发了一种在 3T 下用于质子 MT 实验的窄带γ-ATP 选择性饱和磁化传递序列。
使用γ-ATP 逐渐饱和，在 3T 下测量磷酸肌酸（PCr）和无机磷（Pi）的固有 T 弛豫时间分别为 5.1±0.8s 和 3.0±1.4s。使用γ-ATP 的稳态饱和，在由 10 分钟休息和 10 分钟刺激组成的范式中，在第一次和第二次循环中观察到正向肌酸激酶（CK）准一级反应速率常数 k 分别显著增加 24%±14%和 11%±7%，测量的基线 k 为 0.35±0.04s。在刺激时，未检测到正向 ATP 合酶反应速率、PCr/γ-ATP、Pi/γ-ATP、烟酰胺腺嘌呤二核苷酸/γ-ATP 比或细胞内 pH 的显著变化。
本研究证明了使用功能质子 MRS-MT 来确定 3T 下正向 CK 反应速率变化来研究大脑生物能量学的潜力。磁共振医学 79:22-30,2018。© 2017 作者。磁共振医学由 Wiley 期刊出版公司代表国际磁共振医学学会出版。这是在知识共享署名许可条款下的许可，允许在任何媒介中使用、分发和复制，只要原始作品正确引用。
