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低剂量至高剂量照射后MCF-10A和MCF-7细胞系中miR-155、miR-21和let-7a的表达

miR-155, miR-21, and let-7a Expressions in MCF-10A and MCF-7 Cell Lines after Low to High Dose Irradiation.

作者信息

Zare Afsaneh, Fardid Reza, Tamadon G Holam Hossein, Mosleh-Shirazi Mohammad Amin

机构信息

Department of Radiology, School of Paramedical Sciences, Shiraz University of Medical Sciences, Shiraz, Iran.

Department of Radiology, School of Paramedical Sciences, Shiraz University of Medical Sciences, Shiraz, Iran. Email:

出版信息

Cell J. 2021 Oct;23(5):532-537. doi: 10.22074/cellj.2021.7411. Epub 2021 Oct 30.

DOI:10.22074/cellj.2021.7411
PMID:34837680
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8588820/
Abstract

OBJECTIVE

Ionizing radiation is a tremendous risk factor for cancer development. MicroRNAs (miRNAs) are regulators that utilize cell pathways, which are implicated in human cancer prognosis. In addition, miRNAs respond to anti-cancer therapy and proliferation after irradiation. However, the changes in miRNA expression profiles in response to irradiation have not been comprehensively analysed. The present study was designed to assess potential changes that occur in miRNA expression following irradiation.

MATERIALS AND METHODS

In this experimental study, we used quantitative real-time polymerase chain reaction (qRTPCR) to measure the expressions of miR-155, miR-21, and let-7a in MCF-10A (normal breast cells) and MCF-7 (breast cancer cells) six hours after the cells were exposed to five different irradiation doses (50, 100, 400, 2000, and 4000 mGY).

RESULTS

After irradiation from the low to high doses, we observed an upsurge in miR-155 (more than 100%) expression and reduction in let-7a (more than 87%) expression. However, there was an increase and a reduction in miR-21 expression (more than 100%).

CONCLUSION

Irradiation can play an important role in cancer development in normal breast cells (MCF-10A) at low dose irradiation. However, the results showed little difference at high doses of radiation.

摘要

目的

电离辐射是癌症发生的一个巨大风险因素。微小RNA（miRNA）是利用细胞通路的调节因子，这些通路与人类癌症预后有关。此外，miRNA对放疗和照射后的增殖有反应。然而，尚未全面分析miRNA表达谱对照射的反应变化。本研究旨在评估照射后miRNA表达中发生的潜在变化。

材料与方法

在本实验研究中，我们使用定量实时聚合酶链反应（qRTPCR）来测量MCF-10A（正常乳腺细胞）和MCF-7（乳腺癌细胞）在暴露于五种不同照射剂量（50、100、400、2000和4000毫戈瑞）六小时后miR-155、miR-21和let-7a的表达。

结果

从低剂量到高剂量照射后，我们观察到miR-155表达激增（超过100%），let-7a表达降低（超过87%）。然而，miR-21表达有升有降（超过100%）。

结论

低剂量照射时，辐射在正常乳腺细胞（MCF-10A）的癌症发生中可发挥重要作用。然而，高剂量辐射时结果显示差异不大。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/38d2/8588820/d88fc732eeff/Cell-J-23-532-g05.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/38d2/8588820/3b2c345ecb86/Cell-J-23-532-g01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/38d2/8588820/d8d7fc9bfd1a/Cell-J-23-532-g02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/38d2/8588820/a80a3df494c7/Cell-J-23-532-g03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/38d2/8588820/f085087fec37/Cell-J-23-532-g04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/38d2/8588820/d88fc732eeff/Cell-J-23-532-g05.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/38d2/8588820/3b2c345ecb86/Cell-J-23-532-g01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/38d2/8588820/d8d7fc9bfd1a/Cell-J-23-532-g02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/38d2/8588820/a80a3df494c7/Cell-J-23-532-g03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/38d2/8588820/f085087fec37/Cell-J-23-532-g04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/38d2/8588820/d88fc732eeff/Cell-J-23-532-g05.jpg

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