School of Medicine, Yunnan University, Kunming, Yunnan, China.
State Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, China.
BMC Microbiol. 2021 Nov 27;21(1):328. doi: 10.1186/s12866-021-02390-0.
The spontaneous osteoarthritis (OA) in rhesus macaque is similar to OA in human, which maintains an upright body posture and shows very similar biomechanical properties of bones to humans. At present, there is no good treatment for OA. This study aims to explore relationship between OA and intestinal microbiota, and provide a reference for the treatment of clinical OA.
We collected colonic contents of the 20 rhesus macaque (6-15 years old, female) for intestinal microbiota analysis by metagenomics sequencing, of which 10 were spontaneous OA monkeys and 10 were normal monkeys. Our results showed the diversity of gut microbiota in monkeys with OA was decreased compared to the normal monkeys (p = 0.16). Mollicutes, Tenericutes, Coprobacillus and Faecalitalea may be biomarkers for the monkeys of OA. Lactobacillus found significantly increased in OA monkeys. Prevotella and Ruminococcus were higher in the normal group than OA group. Zinc/manganese transport system permease protein (p = 0.0011) and Cyclopropane-fatty-acyl-phospholipid synthase (p = 0.0012) are a microbiota metabolic pathway related to cartilage production.
Our results indicate that the diversity and composition of intestinal microbiota in monkeys with OA are different compared to the normal monkeys. we have found microbes that may be a biomarker for the diagnosis of osteoarthritis. Functional analysis of the microbiota also predicts cartilage damage in the monkeys with osteoarthritis. Non-human primates are closely related to humans, so this study can provide a reference for the development of drugs for the treatment of OA.
恒河猴自发性骨关节炎（OA）与人 OA 相似，恒河猴维持直立姿势，骨骼的生物力学特性与人非常相似。目前，OA 尚无良好的治疗方法。本研究旨在探讨 OA 与肠道微生物群的关系，为临床 OA 的治疗提供参考。
我们通过宏基因组测序收集了 20 只（6-15 岁，雌性）恒河猴的结肠内容物进行肠道微生物组分析，其中 10 只为自发性 OA 猴，10 只为正常猴。我们的结果表明，OA 猴的肠道微生物多样性与正常猴相比降低（p=0.16）。软支原体、厚壁菌门、粪杆菌属和真杆菌属可能是 OA 猴的生物标志物。OA 猴中乳杆菌明显增加。正常组中普雷沃氏菌属和瘤胃球菌属高于 OA 组。锌/锰转运系统渗透蛋白（p=0.0011）和环丙烷脂肪酸磷脂合成酶（p=0.0012）是与软骨生成相关的微生物代谢途径。
我们的结果表明，OA 猴的肠道微生物多样性和组成与正常猴不同。我们已经发现了一些可能是 OA 诊断标志物的微生物。微生物的功能分析还预测了 OA 猴的软骨损伤。非人类灵长类动物与人密切相关，因此本研究可为 OA 治疗药物的开发提供参考。
