China Pharmaceutical Culture Collection, Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, PR China.
China Pharmaceutical Culture Collection, Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, PR China.
Sci Total Environ. 2022 Feb 25;809:151937. doi: 10.1016/j.scitotenv.2021.151937. Epub 2021 Nov 26.
Kongsfjorden in the high Arctic, a typical Arctic fjord, experienced long-time input of nutrients and pollutants from the remote and local resources, providing a platform for characterizing the diversity and distribution of antibiotic resistance genes (ARGs). However, the microbiome and antibiotic resistome in this pristine marine system have not been well documented. The present study aimed to characterize the diversity and distribution of bacterial communities and associated ARGs in seawater (12 samples) and sediments (13 samples) of Kongsfjorden via metagenomic analysis. In terms of both bacterial community compositions and ARG profiles, the seawater was significantly distinct from sediment. Only 29 ARG subtypes were detected in the Arctic seawater and sediments. Furthermore, three geochemical factors (i.e., longitude, depth, and PO) greatly influenced the bacterial communities in sediment samples, while longitude, depth, and latitude were crucial geochemical factors influencing the ARG profiles in sediment samples. Procrustes analysis revealed a significant correlation between bacterial community compositions and ARG profiles in seawater and sediment samples. Further analysis revealed the metagenome-assembled genomes (MAGs) with ARG subtypes. Overall, our study provides insights into the microbiome and resistome in a pristine Arctic fjord, thereby providing vital information for environmental management.
在高北极的 Kongsfjorden，典型的北极峡湾，长期以来一直受到来自偏远和当地资源的营养物质和污染物的输入，为表征抗生素耐药基因（ARGs）的多样性和分布提供了一个平台。然而，这个原始海洋系统中的微生物组和抗药组尚未得到很好的记录。本研究旨在通过宏基因组分析，描述 Kongsfjorden 海水（12 个样本）和沉积物（13 个样本）中细菌群落和相关抗生素耐药基因（ARGs）的多样性和分布。就细菌群落组成和 ARG 谱而言，海水与沉积物有显著差异。仅在北极海水中检测到 29 种 ARG 亚型。此外，三个地球化学因素（即经度、深度和 PO）极大地影响了沉积物样本中的细菌群落，而经度、深度和纬度是影响沉积物样本中 ARG 谱的关键地球化学因素。普罗克鲁斯分析显示，海水和沉积物样本中的细菌群落组成和 ARG 谱之间存在显著相关性。进一步的分析揭示了具有 ARG 亚型的宏基因组组装基因组（MAGs）。总的来说，我们的研究提供了对原始北极峡湾微生物组和抗药组的深入了解，为环境管理提供了重要信息。
