Susilowati Suherni, Mustofa Imam, Wurlina Wurlina, Triana Indah Norma, Utama Suzanita, Utomo Budi
Division of Veterinary Reproduction, Faculty of Veterinary Medicine, Universitas Airlangga, Kampus C Unair, Mulyorejo, Surabaya 60115, Indonesia.
Vet Med Int. 2021 Nov 18;2021:1470209. doi: 10.1155/2021/1470209. eCollection 2021.
Artificial insemination has proven to be an effective method for increasing population size and genetic quality of Kacang goats. However, innovation is required to maintain the quality of Kacang goat semen in storage. This study aimed to examine the effects of supplementing the 150 kDa protein assumed as IGF-I complex derived from bull seminal plasma in skim milk-egg yolk extender on the quality of Kacang goat sperm stored at 5°C. Twelve ejaculates collected from three Kacang goats were divided into three groups. In the control group (T0), the ejaculates were extended with skim milk-egg yolk only. In the treatment groups (T1 and T2), the ejaculates were extended with skim milk-egg yolk supplemented with the IGF-I complex protein at 12 g and 24 g/100 mL, respectively. The extended semen was stored at 5°C, and the viability, motility, intactness of the plasma membrane, malondialdehyde concentration, and apoptotic sperm percentage were evaluated daily for five days. The results showed that the T1 was the most effective treatment for maintaining Kacang goat semen at a quality acceptable for artificial insemination over five days of storage at 5°C. However, the T0 and T2 groups retained acceptable qualities for only three days at 5°C. It could be concluded that supplementation of 12 g of the 150 kDa protein derived from bull seminal plasma per 100 mL extender successfully extended the life span of Kacang goat sperm for five days.
人工授精已被证明是增加加桑山羊种群数量和遗传质量的有效方法。然而，需要创新方法来保持加桑山羊精液在储存过程中的质量。本研究旨在探讨在脱脂乳-蛋黄稀释液中添加假定为源自公牛精浆的150 kDa蛋白质（胰岛素样生长因子-I复合物）对5°C储存的加桑山羊精子质量的影响。从三只加桑山羊采集的12份精液样本被分为三组。对照组（T0）仅用脱脂乳-蛋黄进行稀释。处理组（T1和T2）分别用添加了12 g/100 mL和24 g/100 mL胰岛素样生长因子-I复合蛋白的脱脂乳-蛋黄进行稀释。稀释后的精液在5°C下储存，连续五天每天评估精子的活力、运动能力、质膜完整性、丙二醛浓度和凋亡精子百分比。结果表明，在5°C下储存五天期间，T1组是保持加桑山羊精液质量以用于人工授精的最有效处理组。然而，T0组和T2组在5°C下仅能保持三天的可接受质量。可以得出结论，每100 mL稀释液中添加12 g源自公牛精浆的150 kDa蛋白质可成功延长加桑山羊精子的寿命至五天。
