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新种和新种,从冰川中分离得到。

sp. nov. and sp. nov., isolated from glaciers.

机构信息

China General Microbiological Culture Collection Center (CGMCC), Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.

Experimental School of Beihang University, Beijing 100083, PR China.

出版信息

Int J Syst Evol Microbiol. 2020 Mar;70(3):1918-1923. doi: 10.1099/ijsem.0.003994.

Abstract

Strains Sr36 and TMT4-23 were isolated from No. 1 glacier in Xinjiang Uygur Autonomous Region and Toumingmengke glacier in Gansu Province, PR China, respectively. They were Gram-stain-positive and rod-shaped micro-organisms. The optimum growth temperature of the two strains was 10-14 °C. Phylogenetic analysis showed that the two strains were related to members of the genus . The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between strain Sr36 and its close relatives Hh15, Hh31, Hh34 and Hh8 were 81.16-87.24 and 28.0-32.5 %, respectively. The ANI and dDDH values between strain TMT4-23 and its close relative 0549 were 81.16 and 22.3 %. The polar lipids of strain Sr36 were diphosphatidylglycerol, phosphatidylglycerol, one unidentified glycolipid and three unidentified lipids. The polar lipids of strain TMT4-23 were diphosphatidylglycerol, phosphatidylglycerol, one unidentified glycolipid, one unidentified phospholipid and six unidentified lipids. The major fatty acids of strain Sr36 were anteiso-C, iso-C, anteiso-C and anteiso-C. The major fatty acids of strain TMT4-23 were anteiso-C, anteiso-C, iso-C, anteiso-C and iso-C. Both strains contained 2,4-diaminobutyric acid and their predominant menaquinone was MK-10. On the basis of the phenotypic, phylogenetic and genotypic data, two novel species sp. nov. (type strain = Sr36=CGMCC 1.9275=NBRC 113797) and sp. nov. (type strain =TMT4-23=CGMCC 1.9556=NBRC 113800) are proposed.

摘要

菌株 Sr36 和 TMT4-23 分别分离自中国新疆维吾尔自治区 1 号冰川和甘肃透明梦柯冰川。它们均为革兰氏阳性、杆状微生物。两株菌的最适生长温度为 10-14°C。系统发育分析表明,两株菌与属内的成员有关。Sr36 菌株与其近亲 Hh15、Hh31、Hh34 和 Hh8 的平均核苷酸同一性(ANI)和数字 DNA-DNA 杂交(dDDH)值分别为 81.16-87.24%和 28.0-32.5%。TMT4-23 菌株与其近亲 0549 的 ANI 和 dDDH 值分别为 81.16%和 22.3%。Sr36 菌株的极性脂包括双磷脂酰甘油、磷脂酰甘油、一种未鉴定的糖脂和三种未鉴定的脂类。TMT4-23 菌株的极性脂包括双磷脂酰甘油、磷脂酰甘油、一种未鉴定的糖脂、一种未鉴定的磷脂和六种未鉴定的脂类。Sr36 菌株的主要脂肪酸为anteiso-C、iso-C、anteiso-C 和 anteiso-C。TMT4-23 菌株的主要脂肪酸为 anteiso-C、anteiso-C、iso-C、anteiso-C 和 iso-C。两株菌均含有 2,4-二氨基丁酸,其主要的menaquinone 为 MK-10。基于表型、系统发育和基因型数据,提出了两个新的物种: sp. nov.(模式株=Sr36=CGMCC 1.9275=NBRC 113797)和 sp. nov.(模式株=TMT4-23=CGMCC 1.9556=NBRC 113800)。

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