China General Microbiological Culture Collection Center (CGMCC), Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
Experimental School of Beihang University, Beijing 100083, PR China.
Int J Syst Evol Microbiol. 2020 Mar;70(3):1918-1923. doi: 10.1099/ijsem.0.003994.
Strains Sr36 and TMT4-23 were isolated from No. 1 glacier in Xinjiang Uygur Autonomous Region and Toumingmengke glacier in Gansu Province, PR China, respectively. They were Gram-stain-positive and rod-shaped micro-organisms. The optimum growth temperature of the two strains was 10-14 °C. Phylogenetic analysis showed that the two strains were related to members of the genus . The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between strain Sr36 and its close relatives Hh15, Hh31, Hh34 and Hh8 were 81.16-87.24 and 28.0-32.5 %, respectively. The ANI and dDDH values between strain TMT4-23 and its close relative 0549 were 81.16 and 22.3 %. The polar lipids of strain Sr36 were diphosphatidylglycerol, phosphatidylglycerol, one unidentified glycolipid and three unidentified lipids. The polar lipids of strain TMT4-23 were diphosphatidylglycerol, phosphatidylglycerol, one unidentified glycolipid, one unidentified phospholipid and six unidentified lipids. The major fatty acids of strain Sr36 were anteiso-C, iso-C, anteiso-C and anteiso-C. The major fatty acids of strain TMT4-23 were anteiso-C, anteiso-C, iso-C, anteiso-C and iso-C. Both strains contained 2,4-diaminobutyric acid and their predominant menaquinone was MK-10. On the basis of the phenotypic, phylogenetic and genotypic data, two novel species sp. nov. (type strain = Sr36=CGMCC 1.9275=NBRC 113797) and sp. nov. (type strain =TMT4-23=CGMCC 1.9556=NBRC 113800) are proposed.
菌株 Sr36 和 TMT4-23 分别分离自中国新疆维吾尔自治区 1 号冰川和甘肃透明梦柯冰川。它们均为革兰氏阳性、杆状微生物。两株菌的最适生长温度为 10-14°C。系统发育分析表明，两株菌与属内的成员有关。Sr36 菌株与其近亲 Hh15、Hh31、Hh34 和 Hh8 的平均核苷酸同一性（ANI）和数字 DNA-DNA 杂交（dDDH）值分别为 81.16-87.24%和 28.0-32.5%。TMT4-23 菌株与其近亲 0549 的 ANI 和 dDDH 值分别为 81.16%和 22.3%。Sr36 菌株的极性脂包括双磷脂酰甘油、磷脂酰甘油、一种未鉴定的糖脂和三种未鉴定的脂类。TMT4-23 菌株的极性脂包括双磷脂酰甘油、磷脂酰甘油、一种未鉴定的糖脂、一种未鉴定的磷脂和六种未鉴定的脂类。Sr36 菌株的主要脂肪酸为anteiso-C、iso-C、anteiso-C 和 anteiso-C。TMT4-23 菌株的主要脂肪酸为 anteiso-C、anteiso-C、iso-C、anteiso-C 和 iso-C。两株菌均含有 2,4-二氨基丁酸，其主要的menaquinone 为 MK-10。基于表型、系统发育和基因型数据，提出了两个新的物种： sp. nov.（模式株=Sr36=CGMCC 1.9275=NBRC 113797）和 sp. nov.（模式株=TMT4-23=CGMCC 1.9556=NBRC 113800）。
