Zhang Xiao, Liu Yang, Luo Chunyang, Zhai Chenjun, Li Zuxi, Zhang Yi, Yuan Tao, Dong Shilei, Zhang Jiyong, Fan Weimin
Department of Orthopedics, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210029, China.
Department of Orthopedics, Yixing People's Hospital, Yixing, Jiangsu 214200, China.
Mater Sci Eng C Mater Biol Appl. 2021 Jan;118:111388. doi: 10.1016/j.msec.2020.111388. Epub 2020 Aug 22.
As cartilage tissue lacks the innate ability to mount an adequate regeneration response, damage to it is detrimental to the quality of life of the subject. The emergence of three-dimensional bioprinting (3DBP) technology presents an opportunity to repair articular cartilage defects. However, widespread adoption of this technique has been impeded by difficulty in preparing a suitable bioink and the toxicity inherent in the chemical crosslinking process of most bioinks. Our objective was to develop a crosslinker-free bioink with the same biological activity as the original cartilage extracellular matrix (ECM) and good mechanical strength. We prepared bioinks containing different concentrations of silk fibroin and decellularized extracellular matrix (SF-dECM bioinks) mixed with bone marrow mesenchymal stem cells (BMSCs) for 3D bioprinting. SF and dECM interconnect with each other through physical crosslinking and entanglement. A porous structure was formed by removing the polyethylene glycol from the SF-dECM bioink. The results showed the SF-dECM construct had a suitable mechanical strength and degradation rate, and the expression of chondrogenesis-specific genes was found to be higher than that of the SF control construct group. Finally, we confirmed that a SF-dECM construct that was designed to release TGF-β3 had the ability to promote chondrogenic differentiation of BMSCs and provided a good cartilage repair environment, suggesting it is an ideal scaffold for cartilage tissue engineering.
由于软骨组织缺乏产生足够再生反应的固有能力，软骨损伤会对患者的生活质量产生不利影响。三维生物打印（3DBP）技术的出现为修复关节软骨缺损提供了契机。然而，由于难以制备合适的生物墨水以及大多数生物墨水化学交联过程中固有的毒性，该技术的广泛应用受到了阻碍。我们的目标是开发一种无交联剂的生物墨水，使其具有与原始软骨细胞外基质（ECM）相同的生物活性和良好的机械强度。我们制备了含有不同浓度丝素蛋白和脱细胞外基质的生物墨水（丝素蛋白-脱细胞外基质生物墨水），并与骨髓间充质干细胞（BMSCs）混合用于3D生物打印。丝素蛋白和脱细胞外基质通过物理交联和缠结相互连接。通过从丝素蛋白-脱细胞外基质生物墨水中去除聚乙二醇形成了多孔结构。结果表明，丝素蛋白-脱细胞外基质构建体具有合适的机械强度和降解速率，并且发现软骨生成特异性基因的表达高于丝素蛋白对照构建体组。最后，我们证实设计用于释放转化生长因子-β3的丝素蛋白-脱细胞外基质构建体具有促进骨髓间充质干细胞软骨分化的能力，并提供了良好的软骨修复环境，表明它是软骨组织工程的理想支架。
