Cho Eun-Jeong, Choi Youngju, Kim Jiyeon, Bae Jun Hyun, Cho Jinkyung, Park Dong-Ho, Kang Ju-Hee, Yoon Jin Hwan, Park Eunmi, Seo Dae Yun, Lee Sukho, Kwak Hyo-Bum
Department of Biomedical Science and Engineering, Inha University, Incheon, Korea.
Institute of Sports & Arts Convergence, Inha University, Incheon, Korea.
Int Neurourol J. 2021 Nov;25(Suppl 2):S47-54. doi: 10.5213/inj.2142334.167. Epub 2021 Nov 30.
The effects of aerobic exercise training on soleus muscle morphology, mitochondria-mediated apoptotic signaling, and atrophy/hypertrophy signaling in ovariectomized rat skeletal muscle were investigated.
Female Sprague-Dawley rats were divided into control (CON), ovariectomy (OVX), and ovariectomy plus exercise (OVX+EX) groups. After ovarian excision, exercise training was performed using a rat treadmill at 20 m/min, 50 min/day, 5 days/week for 12 weeks. Protein levels of mitochondria-mediated apoptotic signaling and atrophy/hypertrophy signaling in the skeletal muscle (soleus) were examined through western immunoblot analysis.
The number of myocytes and myocyte cross-sectional area (CSA) were increased and the extramyocyte space was decreased in the OVX group compared to those in the CON group. However, aerobic exercise training significantly increased myocyte CSA and decreased extramyocyte space in the OVX+EX group compared to those in the OVX group. The protein levels of proapoptotic signaling and muscle atrophy signaling were significantly increased, whereas the protein levels of muscle hypertrophy signaling were significantly decreased in the OVX group compared to that in the CON group. Aerobic exercise training significantly decreased the protein levels of proapoptotic signaling and increased the protein level of antiapoptotic protein in the OVX+EX group compared to that in the OVX group. Aerobic exercise training significantly increased the protein levels of hypertrophy signaling and decreased protein levels of atrophy signaling in the OVX+EX group compared to those in the OVX group.
Treadmill exercise improved estrogen deficiency-induced impairment in skeletal muscle remodeling, mitochondria-mediated apoptotic signaling, and atrophy/hypertrophy signaling in skeletal muscle.
研究有氧运动训练对去卵巢大鼠骨骼肌比目鱼肌形态、线粒体介导的凋亡信号以及萎缩/肥大信号的影响。
将雌性Sprague-Dawley大鼠分为对照组（CON）、去卵巢组（OVX）和去卵巢加运动组（OVX+EX）。卵巢切除后，使用大鼠跑步机以20米/分钟的速度进行运动训练，每天50分钟，每周5天，共12周。通过蛋白质免疫印迹分析检测骨骼肌（比目鱼肌）中线粒体介导的凋亡信号和萎缩/肥大信号的蛋白质水平。
与CON组相比，OVX组的肌细胞数量和肌细胞横截面积（CSA）增加，肌细胞外间隙减小。然而，与OVX组相比，有氧运动训练显著增加了OVX+EX组中的肌细胞CSA并减小了肌细胞外间隙。与CON组相比，OVX组中促凋亡信号和肌肉萎缩信号的蛋白质水平显著增加，而肌肉肥大信号的蛋白质水平显著降低。与OVX组相比，有氧运动训练显著降低了OVX+EX组中促凋亡信号的蛋白质水平并增加了抗凋亡蛋白的蛋白质水平。与OVX组相比，有氧运动训练显著增加了OVX+EX组中肥大信号的蛋白质水平并降低了萎缩信号的蛋白质水平。
跑步机运动改善了雌激素缺乏引起的骨骼肌重塑、线粒体介导的凋亡信号以及骨骼肌萎缩/肥大信号的损伤。
