Department of Biology and Ecology, Faculty of Science, University of Kragujevac, Kragujevac, Serbia.
Department of Chemistry, Faculty of Science, University of Kragujevac, Kragujevac, Serbia.
Drug Chem Toxicol. 2022 Jul;45(4):1915-1922. doi: 10.1080/01480545.2021.2007025. Epub 2021 Nov 29.
In this study, the genotoxic activity of acetone and aqueous extracts of two species of genus Artemisia ( L. and Turra), and possible role of their polyphenolic composition in the observed activities were investigated. Polyphenolic contents were evaluated by high-performance liquid chromatography (HPLC-PDA), while the genotoxic activity was tested using cytokinesis block micronucleus (CBMN) assay on human peripheral blood lymphocytes (PBLs) . HPLC-PDA showed that both extracts were richer in polyphenolic contents than extracts. The acetone extract was the richest of polyphenolic content where we detected six phenolic acids and two flavonoids. CBMN assay showed that aqueous extract of significantly increased micronucleus (MN) frequency in the PBLs treated with all tested concentrations (10, 50, 100, and 250 µg/mL), while did not significantly affect the mean MN frequency. Further, both acetone extracts were genotoxic in all tested concentrations, except the lowest tested (10 µg/mL) of . All tested extracts affected the nuclear division index (NDI) except the aqueous extract ( < 0.05). Based on our results, we can conclude that both acetone and aqueous extracts and acetone extract were genotoxic in PBLs . aqueous extract was not genotoxic and cytotoxic in tested concentrations. We suggest that the aqueous extract of can be used in treatment, which has been confirmed by traditional medicine, but with a high dose of caution and not in high concentrations.
在这项研究中，我们研究了两种蒿属植物（L. 和 Turra）的丙酮和水提物的遗传毒性活性，以及其多酚成分在观察到的活性中的可能作用。多酚含量通过高效液相色谱法（HPLC-PDA）进行评估，而遗传毒性活性则使用人外周血淋巴细胞（PBL）的细胞分裂阻断微核（CBMN）试验进行测试。HPLC-PDA 显示，两种提取物的多酚含量均高于提取物。丙酮提取物是多酚含量最丰富的提取物，其中我们检测到六种酚酸和两种类黄酮。CBMN 试验表明，水提物在所有测试浓度（10、50、100 和 250 μg/mL）下均显著增加了 PBL 中的微核（MN）频率，而提取物则未显著影响 MN 频率的平均值。此外，除了最低测试浓度（10 μg/mL）外，两种丙酮提取物在所有测试浓度下均具有遗传毒性，而水提物则没有。所有测试的提取物均影响核分裂指数（NDI），除了水提物（<0.05）。基于我们的结果，我们可以得出结论，丙酮和水提物以及丙酮提取物均对 PBL 具有遗传毒性。水提物在测试浓度下既没有遗传毒性也没有细胞毒性。我们建议，已被传统医学证实的水提物可以用于治疗，但需要高度谨慎，且不能使用高浓度。
