Comparative study of the genotoxic activity of L. and Turra extracts .

In this study, the genotoxic activity of acetone and aqueous extracts of two species of genus Artemisia ( L. and Turra), and possible role of their polyphenolic composition in the observed activities were investigated. Polyphenolic contents were evaluated by high-performance liquid chromatography (HPLC-PDA), while the genotoxic activity was tested using cytokinesis block micronucleus (CBMN) assay on human peripheral blood lymphocytes (PBLs) . HPLC-PDA showed that both extracts were richer in polyphenolic contents than extracts. The acetone extract was the richest of polyphenolic content where we detected six phenolic acids and two flavonoids. CBMN assay showed that aqueous extract of significantly increased micronucleus (MN) frequency in the PBLs treated with all tested concentrations (10, 50, 100, and 250 µg/mL), while did not significantly affect the mean MN frequency. Further, both acetone extracts were genotoxic in all tested concentrations, except the lowest tested (10 µg/mL) of . All tested extracts affected the nuclear division index (NDI) except the aqueous extract ( < 0.05). Based on our results, we can conclude that both acetone and aqueous extracts and acetone extract were genotoxic in PBLs . aqueous extract was not genotoxic and cytotoxic in tested concentrations. We suggest that the aqueous extract of can be used in treatment, which has been confirmed by traditional medicine, but with a high dose of caution and not in high concentrations.