Lukacs K, Cohen D A, Kaplan A M
Clin Immunol Immunopathol. 1986 Mar;38(3):344-9. doi: 10.1016/0090-1229(86)90244-8.
Human polymorphonuclear leukocytes (PMN) were purified from purified protein derivative (PPD)-immune individuals and assayed for their ability to act as antigen-presenting cells for PPD to autologous purified lymphocytes or as accessory cells in a PHA mitogenic response with autologous lymphocytes. PMNs were very efficient accessory cells in that the PHA mitogenic response was augmented fourfold by the addition of PMNs to the culture. In contrast, PMNs did not appear to present antigen. Accessory cell function by PMNs was HLA unrestricted in that allogeneic PMNs were equally effective accessory cells. Furthermore, the accessory cell function of PMNs appeared to require cell-cell contact, since culture supernatants from PMNs failed to replace intact PMNs in the mitogenic response.
从纯化蛋白衍生物（PPD）免疫个体中纯化出人类多形核白细胞（PMN），并检测其作为PPD抗原呈递细胞作用于自体纯化淋巴细胞的能力，或作为PHA促有丝分裂反应中与自体淋巴细胞一起的辅助细胞的能力。PMN是非常有效的辅助细胞，因为在培养中加入PMN可使PHA促有丝分裂反应增强四倍。相比之下，PMN似乎不呈递抗原。PMN的辅助细胞功能不受HLA限制，因为同种异体PMN同样是有效的辅助细胞。此外，PMN的辅助细胞功能似乎需要细胞间接触，因为PMN的培养上清液在促有丝分裂反应中无法替代完整的PMN。
