Haar D, Heron I
Scand J Immunol. 1982 Nov;16(5):403-11. doi: 10.1111/j.1365-3083.1982.tb00740.x.
Autologous monocytes are required for an optimal lymphocyte proliferative response to purified protein derivate of tuberculin (PPD) in vitro and for a mixed lymphocyte culture induced by alloantigens. In the proliferative response to PPD we found that autologous monocytes could be replaced with HLA-DR-compatible monocytes and partly with HLA-DR semi-identical. In spite of a statistically significant difference between autologous and HLA-DR disparate monocytes in their cooperative capacity with PPD-stimulated lymphocytes, replacement in nearly one third of the cases was possible. These findings were supported by more detailed studies in which increasing numbers of allogenic and autologous monocytes were added to the isolated lymphocytes in the presence of PPD. It is concluded that the serologically defined HLA-DR antigens alone give insufficient information of the restriction elements controlling the PPD-stimulated lymphocyte-monocyte interactions.
自体单核细胞对于体外对结核菌素纯蛋白衍生物（PPD）的最佳淋巴细胞增殖反应以及同种异体抗原诱导的混合淋巴细胞培养是必需的。在对PPD的增殖反应中，我们发现自体单核细胞可以被HLA - DR兼容的单核细胞替代，部分也可被HLA - DR半相同的单核细胞替代。尽管自体单核细胞与HLA - DR不同的单核细胞在与PPD刺激的淋巴细胞的协同能力上存在统计学上的显著差异，但在近三分之一的病例中仍有可能进行替代。在有PPD存在的情况下，将越来越多的同种异体和自体单核细胞添加到分离的淋巴细胞中的更详细研究支持了这些发现。得出的结论是，仅血清学定义的HLA - DR抗原不足以提供控制PPD刺激的淋巴细胞 - 单核细胞相互作用的限制元件的信息。
