• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

脊髓EZH2参与大鼠骨癌疼痛发展的证据。

Evidence of the Involvement of Spinal EZH2 in the Development of Bone Cancer Pain in Rats.

作者信息

Chen Haoming, Yu Jianmang, Hang Lihua, Li Shuai, Lu Weikang, Xu Zhenkai

机构信息

Department of Anesthesiology, Kunshan First People's Hospital Affiliated to Jiangsu University, Kunshan, Jiangsu, 215300, People's Republic of China.

出版信息

J Pain Res. 2021 Nov 23;14:3593-3600. doi: 10.2147/JPR.S331114. eCollection 2021.

DOI:10.2147/JPR.S331114
PMID:34849017
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8627314/
Abstract

INTRODUCTION

Bone cancer pain (BCP) seriously affects the quality of life of patients with advanced cancer, but effective treatment methods are lacking. This study mainly investigates the role of EZH2 in a well-established BCP model induced by Walker 256 breast cancer cells in rats.

METHODS

Female Sprague-Dawley rats of the same age weighing approximately 160 g were selected for the experiment. The BCP model was established by injecting inactivated Walker 256 breast cancer cells into the tibia. von Frey filaments were used to measure the paw withdrawal threshold, and bone destruction in the rat was observed using x-ray. The spinal EZH2 and H3K27Tm levels were measured using Western blotting and RT-qPCR analysis. Intrathecal injection of an EZH2 inhibitor was performed to examine the role of EZH2 in trigeminal BCP.

RESULTS

Experimental results showed that injecting Walker 256 breast cancer cells into the tibia induced bone cancer pain. Spinal EZH2 and H3K27Tm levels were significantly increased over time in BCP rats. An intrathecal injection of 3-deazaneplanocin A (DZNep), a selective EZH2 inhibitor, downregulated the expression of EZH2 and attenuate the BCP-induced mechanical allodynia state.

CONCLUSION

Intrathecal injection of DZNep relieve bone cancer pain in rats. EZH2 expressed in spinal cord tissue may be involved in the process of bone cancer pain in rats.

摘要

引言

骨癌疼痛（BCP）严重影响晚期癌症患者的生活质量，但缺乏有效的治疗方法。本研究主要探讨EZH2在Walker 256乳腺癌细胞诱导的大鼠骨癌疼痛模型中的作用。

方法

选取年龄相同、体重约160 g的雌性Sprague-Dawley大鼠进行实验。通过将灭活的Walker 256乳腺癌细胞注入胫骨建立骨癌疼痛模型。使用von Frey细丝测量爪部撤离阈值，并通过X射线观察大鼠的骨质破坏情况。采用蛋白质免疫印迹法和逆转录定量聚合酶链反应分析测量脊髓EZH2和H3K27Tm水平。进行鞘内注射EZH2抑制剂以研究EZH2在三叉神经骨癌疼痛中的作用。

结果

实验结果表明，将Walker 256乳腺癌细胞注入胫骨可诱导骨癌疼痛。在骨癌疼痛大鼠中，脊髓EZH2和H3K27Tm水平随时间显著升高。鞘内注射选择性EZH2抑制剂3-去氮杂氮胞苷（DZNep）可下调EZH2的表达，并减轻骨癌疼痛诱导的机械性异常疼痛状态。

结论

鞘内注射DZNep可减轻大鼠骨癌疼痛。脊髓组织中表达的EZH2可能参与大鼠骨癌疼痛的过程。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/10e0/8627314/5aaa443b2b5f/JPR-14-3593-g0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/10e0/8627314/282ca7a1888c/JPR-14-3593-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/10e0/8627314/c03089538b28/JPR-14-3593-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/10e0/8627314/fd491d854688/JPR-14-3593-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/10e0/8627314/5aaa443b2b5f/JPR-14-3593-g0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/10e0/8627314/282ca7a1888c/JPR-14-3593-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/10e0/8627314/c03089538b28/JPR-14-3593-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/10e0/8627314/fd491d854688/JPR-14-3593-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/10e0/8627314/5aaa443b2b5f/JPR-14-3593-g0004.jpg

相似文献

1
Evidence of the Involvement of Spinal EZH2 in the Development of Bone Cancer Pain in Rats.脊髓EZH2参与大鼠骨癌疼痛发展的证据。
J Pain Res. 2021 Nov 23;14:3593-3600. doi: 10.2147/JPR.S331114. eCollection 2021.
2
EZH2 regulates spinal neuroinflammation in rats with neuropathic pain.EZH2调节神经性疼痛大鼠的脊髓神经炎症。
Neuroscience. 2017 May 4;349:106-117. doi: 10.1016/j.neuroscience.2017.02.041. Epub 2017 Feb 28.
3
Suppression of histone deacetylases by SAHA relieves bone cancer pain in rats via inhibiting activation of glial cells in spinal dorsal horn and dorsal root ganglia.SAHA 通过抑制脊髓背角和背根神经节胶质细胞的激活缓解大鼠骨癌痛。
J Neuroinflammation. 2020 Apr 22;17(1):125. doi: 10.1186/s12974-020-01740-5.
4
Evidence of the involvement of spinal αB-crystallin in the maintenance of bone cancer pain in rats.脊髓αB-晶体蛋白参与大鼠骨癌痛维持的证据。
Pharmacol Rep. 2020 Feb;72(1):208-213. doi: 10.1007/s43440-019-00052-7. Epub 2020 Jan 8.
5
LncRNA 51325 Alleviates Bone Cancer Induced Hyperalgesia Through Inhibition of Pum2.长链非编码RNA 51325通过抑制Pum2减轻骨癌诱导的痛觉过敏。
J Pain Res. 2024 Jan 16;17:265-284. doi: 10.2147/JPR.S446635. eCollection 2024.
6
The short interference RNA (siRNA) targeting NMUR2 relieves nociception in a bone cancer pain model of rat through PKC-ERK and PI3K-AKT pathways.靶向 NMUR2 的短干扰 RNA (siRNA) 通过 PKC-ERK 和 PI3K-AKT 通路缓解大鼠骨癌痛模型中的痛觉。
Biochem Biophys Res Commun. 2019 May 7;512(3):616-622. doi: 10.1016/j.bbrc.2019.03.067. Epub 2019 Mar 23.
7
Analgesic Effect of Intrathecal Administration of Chemokine Receptor CCR2 Antagonist is Related to Change in Spinal NR2B, nNOS, and SIGIRR Expression in Rat with Bone Cancer Pain.鞘内注射趋化因子受体CCR2拮抗剂对骨癌痛大鼠的镇痛作用与脊髓NR2B、nNOS和SIGIRR表达变化有关。
Cell Biochem Biophys. 2015 Jun;72(2):611-6. doi: 10.1007/s12013-014-0510-7.
8
Metformin Attenuates Bone Cancer Pain by Reducing TRPV1 and ASIC3 Expression.二甲双胍通过降低TRPV1和ASIC3的表达减轻骨癌疼痛。
Front Pharmacol. 2021 Aug 4;12:713944. doi: 10.3389/fphar.2021.713944. eCollection 2021.
9
The analgesic effects of triptolide in the bone cancer pain rats via inhibiting the upregulation of HDACs in spinal glial cells.雷公藤内酯醇通过抑制脊髓胶质细胞中 HDACs 的上调发挥抗骨癌痛作用。
J Neuroinflammation. 2017 Nov 2;14(1):213. doi: 10.1186/s12974-017-0988-1.
10
The TRPA1 Channel Mediates Mechanical Allodynia and Thermal Hyperalgesia in a Rat Bone Cancer Pain Model.TRPA1通道在大鼠骨癌疼痛模型中介导机械性异常性疼痛和热痛觉过敏。
Front Pain Res (Lausanne). 2021 Mar 22;2:638620. doi: 10.3389/fpain.2021.638620. eCollection 2021.

引用本文的文献

1
Syringetin relieves bone cancer pain in rats induced by breast cancer cells through the ESR1/PRDM2 axis.杨梅黄素通过ESR1/PRDM2轴缓解乳腺癌细胞诱导的大鼠骨癌疼痛。
Korean J Physiol Pharmacol. 2025 Jul 1;29(4):497-508. doi: 10.4196/kjpp.24.303. Epub 2025 Apr 11.
2
Pathology of pain and its implications for therapeutic interventions.疼痛的病理学及其对治疗干预的影响。
Signal Transduct Target Ther. 2024 Jun 8;9(1):155. doi: 10.1038/s41392-024-01845-w.

本文引用的文献

1
Distinct Gene Expression Patterns of Ion Channels and Cytokines in Rat Primary Sensory Neurons During Development of Bone Cancer and Cancer Pain.大鼠原发性感觉神经元在骨癌及癌痛发生发展过程中离子通道和细胞因子的独特基因表达模式
Front Mol Neurosci. 2021 May 6;14:665085. doi: 10.3389/fnmol.2021.665085. eCollection 2021.
2
Calcitonin gene-related peptide regulates spinal microglial activation through the histone H3 lysine 27 trimethylation via enhancer of zeste homolog-2 in rats with neuropathic pain.降钙素基因相关肽通过增强子结合蛋白 2 介导的组蛋白 H3 赖氨酸 27 三甲基化调控神经病理性疼痛大鼠脊髓小胶质细胞的激活。
J Neuroinflammation. 2021 May 21;18(1):117. doi: 10.1186/s12974-021-02168-1.
3
The Spinal α7-Nicotinic Acetylcholine Receptor Contributes to the Maintenance of Cancer-Induced Bone Pain.
脊髓α7-烟碱型乙酰胆碱受体有助于维持癌症诱导的骨痛。
J Pain Res. 2021 Feb 15;14:441-452. doi: 10.2147/JPR.S286321. eCollection 2021.
4
Pain Mechanism in Rheumatoid Arthritis: From Cytokines to Central Sensitization.类风湿关节炎的疼痛机制：从细胞因子到中枢敏化。
Mediators Inflamm. 2020 Sep 12;2020:2076328. doi: 10.1155/2020/2076328. eCollection 2020.
5
Modulation of Rat Cancer-Induced Bone Pain is Independent of Spinal Microglia Activity.大鼠癌症诱导性骨痛的调节与脊髓小胶质细胞活性无关。
Cancers (Basel). 2020 Sep 24;12(10):2740. doi: 10.3390/cancers12102740.
6
Increased EZH2 Levels in Anterior Cingulate Cortex Microglia Aggravate Neuropathic Pain by Inhibiting Autophagy Following Brachial Plexus Avulsion in Rats.大鼠臂丛损伤后，前扣带皮层小胶质细胞中 EZH2 水平升高通过抑制自噬加重神经病理性疼痛。
Neurosci Bull. 2020 Jul;36(7):793-805. doi: 10.1007/s12264-020-00502-w. Epub 2020 Apr 28.
7
Pulsed radiofrequency alleviated neuropathic pain by down-regulating the expression of substance P in chronic constriction injury rat model.脉冲射频通过下调慢性缩窄性损伤大鼠模型中 P 物质的表达缓解神经病理性疼痛。
Chin Med J (Engl). 2020 Jan 20;133(2):190-197. doi: 10.1097/CM9.0000000000000619.
8
PRC2.1 and PRC2.2 Synergize to Coordinate H3K27 Trimethylation.PRC2.1 和 PRC2.2 协同作用以协调 H3K27 三甲基化。
Mol Cell. 2019 Nov 7;76(3):437-452.e6. doi: 10.1016/j.molcel.2019.08.012. Epub 2019 Sep 11.
9
Dissecting the role of H3K27 acetylation and methylation in PRC2 mediated control of cellular identity.解析 H3K27 乙酰化和甲基化在 PRC2 介导的细胞身份控制中的作用。
Nat Commun. 2019 Apr 11;10(1):1679. doi: 10.1038/s41467-019-09624-w.
10
miR-124-3p attenuates neuropathic pain induced by chronic sciatic nerve injury in rats via targeting EZH2.miR-124-3p 通过靶向 EZH2 减轻大鼠慢性坐骨神经损伤引起的神经病理性疼痛。
J Cell Biochem. 2019 Apr;120(4):5747-5755. doi: 10.1002/jcb.27861. Epub 2018 Nov 2.