Ma Qingzhou, Wu Haiyan, Geng Yuehua, Li Qiang, Zang Rui, Guo Yashuang, Xu Chao, Zhang Meng
Department of Plant Pathology, Henan Agricultural University, Zhengzhou, Henan, China.
Analytical Instrument Center, Henan Agricultural University, Zhengzhou, Henan, China.
Comput Struct Biotechnol J. 2021 Nov 3;19:5987-5999. doi: 10.1016/j.csbj.2021.11.002. eCollection 2021.
, the causal agent of an extensive range of plant diseases worldwide, is a momentous fungus with diverse lifestyles and rich in intraspecies variations. In the present study, a total of 56 mitochondrial genomes of were assembled (except two available online) and analyzed, of which 16 mitogenomes were newly sequenced here. All these circular mitochondrial DNA (mtDNA) molecules, ranging from 39,223 bp to 45,786 bp in length, comprised the same set of 13 core protein-coding genes (PCGs), two rRNAs and 27 tRNAs arranged in identical order. Across the above conserved genes, had the largest genetic distance between different isolates and was possibly subjected to positive selection pressure. Comparative mitogenomic analysis indicated that seven group I (IB, IC1, and IC2) introns with a length range of 1013-1876 bp were differentially inserted in three core PCGs (, , and ), resulting in the varied mitogenome sizes among isolates. In combination with dynamic distribution of the introns, a well-supported mitogenome-wide phylogeny of the 56 isolates revealed eight phylogenetic groups, which only had weak correlations with host range and toxin class. Different groups of isolates exhibited obvious differences in length and GC content of some genes, while a degree of variance in codon usage and tRNA structure was also observed. This research served as the first report on mitogenomic comparisons within , and could provide new insights into its intraspecific microevolution and genetic diversity.
作为全球范围内多种植物病害的致病因子，是一种具有多种生活方式且种内变异丰富的重要真菌。在本研究中，共组装（除两个可在线获取的之外）并分析了56个的线粒体基因组，其中16个线粒体基因组是在此新测序的。所有这些环状线粒体DNA（mtDNA）分子长度在39,223 bp至45,786 bp之间，包含相同的一组13个核心蛋白质编码基因（PCGs）、两个rRNA和27个tRNA，它们以相同顺序排列。在上述保守基因中，不同分离株之间的遗传距离最大，可能受到正选择压力。比较线粒体基因组分析表明，7个长度范围为1013 - 1876 bp的I组（IB、IC1和IC2）内含子差异插入到三个核心PCGs（、和）中，导致分离株之间线粒体基因组大小不同。结合内含子的动态分布，对56个分离株进行的全基因组线粒体系统发育分析显示出8个系统发育组，它们与寄主范围和毒素类别仅具有微弱相关性。不同组的分离株在某些基因的长度和GC含量上表现出明显差异，同时在密码子使用和tRNA结构上也观察到一定程度的差异。本研究是关于内线粒体基因组比较的首次报道，可为其种内微进化和遗传多样性提供新的见解。
