Zhang Yuchen, Zhang Xiangdong, Jin Xiaoxuan, Zhang Pan, Liu Kaiyang, Yao Yao, Ru Jiangjiang, Li Yibao, Xu Mimi, Lu Feng, He Yunfan, Gao Jianhua
Department of Plastic Surgery, Nanfang Hospital, Southern Medical University, 1838 Guangzhou North Road, Guangzhou, Guangdong, China.
Aesthet Surg J. 2022 Apr 12;42(5):NP337-NP350. doi: 10.1093/asj/sjab386.
Skin filler is an option for treating skin aging and wrinkles; however, currently used fillers are limited by poor biocompatibility, rapid degradation, and possible hypersensitivity reactions. Autologous adipose tissue-derived products have been recognized as promising options for skin rejuvenation.
This study aimed to develop a novel adipose-derived product for skin filling.
Adipose collagen fragment (ACF) was prepared through pulverization, filtration, and centrifugation. The macrography, structure, types of collagen, and cell viability of ACF were evaluated by immunostaining, western blotting, and cell culture assays. ACF, nanofat, and phosphate-buffered saline (9 spots/side, 0.01 mL/spot) were intradermally injected in the dorsal skin of 36 female BALB/c nude mice; the skin filling capacity and the collagen remodeling process were then investigated. Twenty-one female patients with fine rhytides in the infraorbital areas were enrolled and received clinical applications of ACF treatment. Therapeutic effects and patients' satisfaction scores were recorded.
The mean [standard deviation] yield of ACF from 50 mL of Coleman fat was 4.91 [0.25] mL. ACF contained nonviable cells and high levels of collagen I, collagen IV, and laminin. Fibroblasts and procollagen significantly increased in ACF and ACF-treated dermis (P < 0.05). Overall, 85.7% of patients were satisfied with the therapy results, and no infections, injection site nodules, or other unwanted side effects were observed.
ACF significantly improved dermal thickness and collagen synthesis and may serve as a potential autologous skin filler.
皮肤填充剂是治疗皮肤老化和皱纹的一种选择；然而，目前使用的填充剂受到生物相容性差、快速降解以及可能的过敏反应的限制。自体脂肪组织衍生产品已被认为是皮肤年轻化的有前景的选择。
本研究旨在开发一种用于皮肤填充的新型脂肪衍生产品。
通过粉碎、过滤和离心制备脂肪胶原片段（ACF）。通过免疫染色、蛋白质印迹和细胞培养试验评估ACF的宏观图像、结构、胶原类型和细胞活力。将ACF纳米脂肪和磷酸盐缓冲盐水（每侧9个点，每个点0.01 mL）皮内注射到36只雌性BALB/c裸鼠的背部皮肤中；然后研究皮肤填充能力和胶原重塑过程。招募了21名眶下区域有细纹的女性患者，并接受了ACF治疗的临床应用。记录治疗效果和患者满意度评分。
从50 mL科尔曼脂肪中获得的ACF的平均[标准差]产量为4.91 [0.25] mL。ACF含有无活力细胞以及高水平的I型胶原、IV型胶原和层粘连蛋白。ACF和ACF处理的真皮中成纤维细胞和前胶原显著增加（P < 0.05）。总体而言，85.7%的患者对治疗结果满意，未观察到感染、注射部位结节或其他不良副作用。
ACF显著改善了真皮厚度和胶原合成，可能作为一种潜在的自体皮肤填充剂。
