University of Science & Technology (UST), KIOM Campus, Korean Convergence Medicine Major, Daejeon 34054, Republic of Korea.
Herbal Medicine Resources Research Center, Korea Institute of Oriental Medicine, Jeollanam-do 58245, Republic of Korea.
G3 (Bethesda). 2021 Oct 19;11(11). doi: 10.1093/g3journal/jkab246.
The new yeast Metschnikowia persimmonesis KCTC 12991BP (KIOM G15050 strain) exhibits strong antimicrobial activity against some pathogens. This activity may be related to the medicinal profile of secondary metabolites that could be found in the genome of this species. Therefore, to explore its future possibility of producing some beneficial activities, including medicinal ability, we report high-quality whole-genome assembly of M. persimmonesis produced by PacBio RS II sequencer. The final draft assembly consisted of 16 scaffolds with GC content of 45.90% and comprised a fairly complete set (82.8%) of BUSCO result using Saccharomycetales lineage data set. The total length of the genome was 16.473 Mb, with a scaffold N50 of 1.982 Mb. Annotation of the M. persimmonesis genome revealed presence of 7029 genes and 6939 functionally annotated proteins. Based on the analysis of phylogenetic relationship and the average nucleotide identities, M. persimmonesis was proved to a novel species within the Metschnikowia genus. This finding is expected to significantly contribute to the discovery of high-value natural products from M. persimmonesis as well as for genome biology and evolution comparative analysis within Metschnikowia species.
新的酵母米氏毕赤酵母 KCTC 12991BP（KIOM G15050 株）对一些病原体表现出强烈的抗菌活性。这种活性可能与该物种基因组中发现的次生代谢物的药用特征有关。因此，为了探索其未来产生一些有益活性的可能性，包括药用能力，我们报告了由 PacBio RS II 测序仪产生的米氏毕赤酵母的高质量全基因组组装。最终的草图组装由 16 个支架组成，GC 含量为 45.90%，使用 Saccharomycetales 谱系数据集，BUSCO 结果相当完整（82.8%）。基因组的总长度为 16.473Mb，支架 N50 为 1.982Mb。米氏毕赤酵母基因组的注释显示存在 7029 个基因和 6939 个功能注释蛋白。基于系统发育关系和平均核苷酸同一性的分析，证明米氏毕赤酵母是毕赤酵母属内的一个新种。这一发现有望为从米氏毕赤酵母中发现高价值天然产物以及在毕赤酵母属内进行基因组生物学和进化比较分析做出重要贡献。
