Brito-Babapulle V, Pittman S, Melo J V, Parreira L, Catovsky D
Leuk Res. 1986;10(2):131-8. doi: 10.1016/0145-2126(86)90035-4.
Leukaemic cells from 19 patients with hairy cell leukaemia (HCL), characterised by morphological, immunological and ultrastructural criteria, were investigated for chromosome abnormalities after stimulation with B-cell mitogens (Pokeweed mitogen (PWM), lipopolysaccharide B and EBV). The cells from all cases had a B-cell phenotype and in each case only a single light chain type was expressed on the membrane of the cells. Only 15 patients with adequate metaphases are included in this study. Clonal chromosome abnormalities were observed in 12 patients of which five had a 14q + involving q32. Of the remaining 3 cases 1 had nonclonal abnormalities and 2 had normal karyotypes. The donor chromosomes were identified in 3 cases and were found to be 9, 18 and 22. An interstitial rearrangement of chromosome 14 involving band q22 was seen in 2 cases and a deletion of chromosome 14 at q24 in 1 case. Amongst other chromosome abnormalities 12p was involved in 4 cases, 12q in 2 cases and chromosomes 7 and 22 in 3 cases each. The significance of the abnormalities has been discussed in relation to sites of cellular oncogenes. Our study demonstrates that chromosome abnormalities common to other B-cell disorders are present in HCL.
根据形态学、免疫学和超微结构标准，对19例毛细胞白血病（HCL）患者的白血病细胞进行了研究，这些细胞在用B细胞有丝分裂原（商陆有丝分裂原（PWM）、脂多糖B和EB病毒）刺激后检测染色体异常情况。所有病例的细胞均具有B细胞表型，且每个病例中细胞表面仅表达单一轻链类型。本研究仅纳入了15例有足够中期分裂相的患者。12例患者观察到克隆性染色体异常，其中5例有涉及q32的14q + 。其余3例中，1例有非克隆性异常，2例核型正常。3例鉴定出供体染色体，分别为9号、18号和22号。2例可见涉及14号染色体q22带的中间重排，1例可见14号染色体q24处缺失。在其他染色体异常中，4例涉及12p，2例涉及12q，3例分别涉及7号和22号染色体。已结合细胞癌基因位点讨论了这些异常的意义。我们的研究表明，HCL中存在其他B细胞疾病常见的染色体异常。
