Ohyashiki K, Ohyashiki J H, Takeuchi J, Han T, Henderson E S, Sandberg A A
Cancer Genet Cytogenet. 1987 Jan;24(1):109-17. doi: 10.1016/0165-4608(87)90087-2.
Cytogenetic analyses were performed on cells from 17 patients with hairy cell leukemia stimulated with polyclonal B-cell activators (in 155 different cultures). No mitosis was obtained in samples from four cases (23.5%). Of 14 bone marrows, four (28.6%) showed mitoses, two with clonal abnormalities. All four samples from the spleen had mitoses with four clonal changes; eight of 13 (37.5%) blood samples had mitoses with three clonal changes. Of the polyclonal B-cell activators (PBA), lipopolysaccharide and protein A seemed to be effective for the detection of clonal abnormalities in hairy cell leukemia. Among the clonal aberrations, chromosomes #3, #10, and #17 were affected in two cases each; frequent numerical changes were monosomies of #10 and #17 and structural changes were deletions at band 3p21 (two cases), 6q-, and der(9)t(9;?)(p22;?). The chromosomal bands involved in structural changes were close to accepted constitutive fragile sites.
对17例毛细胞白血病患者经多克隆B细胞激活剂刺激后的细胞进行了细胞遗传学分析（共155种不同培养物）。4例患者（23.5%）的样本未获得有丝分裂。14例骨髓样本中，4例（28.6%）出现有丝分裂，其中2例有克隆异常。所有4例脾脏样本均出现有丝分裂，伴有4种克隆改变；13例血液样本中有8例（37.5%）出现有丝分裂，伴有3种克隆改变。在多克隆B细胞激活剂（PBA）中，脂多糖和蛋白A似乎对检测毛细胞白血病中的克隆异常有效。在克隆畸变中，3号、10号和17号染色体各有2例受累；常见的数目改变是10号和17号染色体单体，结构改变是3p21带缺失（2例）、6号染色体长臂缺失以及9号衍生染色体t(9;?)(p22;?)。结构改变所涉及的染色体带接近公认的结构型脆性位点。
