Petit C, Gilbert M, Sommé G, Leclercq L, Mazié J C, Dorf M E, Thèze J
Mol Immunol. 1982 Sep;19(9):1139-47. doi: 10.1016/0161-5890(82)90324-8.
An anti-idiotypic antiserum was raised in a rabbit against a pool of purified F.344 rat anti-GAT antibodies. GAT-13, the idiotype defined by this serum, is present in all F.344 anti-GAT sera from primary and secondary anti-GAT responses. Anti-GAT sera of 13 inbred rat strains, with different RT1 haplotypes and with different heavy- and light-chain allotypes, all express idiotypic determinants cross-reacting with GAT-13. Thus, like in mice anti-GAT antibodies from rats express public idiotypic determinants. The anti-idiotypic serum also recognizes a highly conserved idiotypic specificity present on mouse and guinea-pig anti-GAT antibodies. The mouse, rat and guinea-pig express a similar highly conserved idiotypic specificity after immunization with GAT. All anti-GAT antibodies from the mouse and guinea-pig bear this idiotypic specificity. These results confirm the existence in the anti-GAT response of interspecies cross-reactive idiotypic determinants.
用纯化的F.344大鼠抗谷氨酸脱羧酶（GAT）抗体混合物在兔体内制备了抗独特型抗血清。由该血清定义的独特型GAT-13存在于初次和二次抗GAT反应的所有F.344抗GAT血清中。13种具有不同RT1单倍型以及不同重链和轻链同种异型的近交系大鼠的抗GAT血清，均表达与GAT-13交叉反应的独特型决定簇。因此，与小鼠一样，大鼠的抗GAT抗体也表达公共独特型决定簇。该抗独特型血清还识别存在于小鼠和豚鼠抗GAT抗体上的高度保守的独特型特异性。在用GAT免疫后，小鼠、大鼠和豚鼠表达相似的高度保守的独特型特异性。小鼠和豚鼠的所有抗GAT抗体都具有这种独特型特异性。这些结果证实了在抗GAT反应中存在种间交叉反应性独特型决定簇。
