School of Life and Environmental Science, Wenzhou University, Chashan, Wenzhou, 325035, People's Republic of China.
School of Environmental Science and Engineering, Shanghai Jiao Tong University, Shanghai, 200240, Minhang, People's Republic of China.
Sci Rep. 2021 Dec 1;11(1):23234. doi: 10.1038/s41598-021-02693-2.
To enhance the reducing sugar yield in enzymatic hydrolysis, various factors (NaOH concentration, solid content and pre-treatment time) that affect the pre-treatment process were investigated and evaluated based on the reducing sugar yield of the subsequent enzymatic hydrolysis. The enzymatic hydrolysis was based on the cellulase from Trichoderma reesi ATCC 26921, the optimum NaOH pre-treatment conditions were an NaOH concentration of 1.0% (w/w), a solid content of 5.0% (w/v) and a pre-treatment time of 60 min. Various parameters that affect the enzymatic hydrolysis of wheat straw, including the solid content, enzyme loading, pH and hydrolysis time, were investigated and optimized through a Box-Behnken design and response surface methodology. The predicted optimum conditions for enzymatic hydrolysis were a solid content of 8.0% (w/v), an enzyme loading of 35 FPU/g substrate, a temperature of 50 °C, a pH of 5.3 and a hydrolysis time of 96 h. The experimental result showed that the maximum reducing sugar yield was 60.73% (53.35% higher than the wheat straw without NaOH pre-treatment), which is in accordance with the predicted conditions.
为了提高酶解过程中的还原糖得率，基于后续酶解的还原糖得率，研究并评估了影响预处理过程的各种因素（NaOH 浓度、固含量和预处理时间）。酶解基于里氏木霉 ATCC 26921 的纤维素酶，NaOH 预处理的最佳条件为：NaOH 浓度 1.0%（w/w），固含量 5.0%（w/v），预处理时间 60 min。通过 Box-Behnken 设计和响应面法研究并优化了影响小麦秸秆酶解的各种参数，包括固含量、酶用量、pH 值和水解时间。酶解的预测最佳条件为：固含量 8.0%（w/v），酶用量 35 FPU/g 底物，温度 50°C，pH 值 5.3，水解时间 96 h。实验结果表明，最大还原糖得率为 60.73%（比未经 NaOH 预处理的小麦秸秆提高了 53.35%），与预测条件相符。
