College of Marine Science and Biological Engineering, Qingdao University of Science and Technology, Qingdao, 266071, China.
Key Laboratory of Marine Eco-Environmental Science and Technology, First Institute of Oceanography, Ministry of Natural Resources, Qingdao, 266061, China.
Environ Sci Pollut Res Int. 2022 Apr;29(18):26821-26834. doi: 10.1007/s11356-021-17826-3. Epub 2021 Dec 2.
In this study, sediments from eight sites were collected from hydrothermal areas (e.g., the Tiancheng, Tianzuo, and Longqi hydrothermal areas) and non-hydrothermal area on the Southwest Mid-Indian Ocean Ridge. Using crude oil as the only carbon and energy source, 162 strains of culturable oil-degrading bacteria were isolated and obtained. The rate of oil degradation of the consortia was 39.48-46.00% in hydrothermal and non-hydrothermal areas. High-throughput sequencing found that the alpha diversity indices (e.g., Shannon and Simpson) of the communities in hydrothermal areas were higher than those in non-hydrothermal area. The species diversities of the oil-degrading bacteria were different among different hydrothermal areas. The composition of the oil-degrading bacterial species in the Tianzuo hydrothermal area tended to be more similar to that in the non-hydrothermal area. This similarity is attributed to the changes in the bacterial community that followed the cessation of hydrothermal vent eruptions at this site. The Alphaproteobacteria abundance of the oil-degrading bacteria was significantly different in oil-degrading bacteria between the hydrothermal and non-hydrothermal areas.
在这项研究中，从西南印度洋中脊热液区（如天成、天祚和龙旗热液区）和非热液区采集了沉积物。使用原油作为唯一的碳源和能源，从这些沉积物中分离和获得了 162 株可培养的石油降解菌。在热液区和非热液区，生物群落的石油降解率分别为 39.48%-46.00%。高通量测序发现，热液区群落的 alpha 多样性指数（如 Shannon 和 Simpson）高于非热液区。不同热液区的石油降解菌的物种多样性不同。天祚热液区石油降解菌的物种组成倾向于与非热液区更相似。这种相似性归因于该地点热液喷口喷发停止后，细菌群落的变化。热液区和非热液区石油降解菌的 Alpha 变形菌丰度存在显著差异。
