School of Life Sciences, University of Technology Sydney, Ultimo, NSW, Australia.
Methods Mol Biol. 2022;2402:81-92. doi: 10.1007/978-1-0716-1843-1_7.
Swept frequency electrical impedance spectroscopy (EIS) can be used in conjunction with tethered bilayer lipid membranes to monitor the membrane permeability of ions in real-time (Deplazes et al. J Phys Chem Lett 11:6353-6358, 2020). Conductance readings, as determined by EIS, are a measure of the ability of ions to be transported across membranes. Recording the change in conductance as a function of cation concentration and a comparison between a range of cations permits conclusions to be made about the specificity of cation transport through pores. An estimate for upper pore size and cation selectivity of ion channels can be established using this method.
扫频交流阻抗谱（EIS）可与连接双层脂质膜联用，实时监测离子的膜通透性（Deplazes 等人，J Phys Chem Lett 11:6353-6358, 2020）。EIS 测定的电导率读数是衡量离子跨膜运输能力的指标。记录电导率随阳离子浓度的变化，并对一系列阳离子进行比较，可以得出关于阳离子通过孔隙的特异性的结论。使用这种方法可以估算离子通道的孔径上限和阳离子选择性。
