Fujiwara H, Tsuyuguchi I
Chest. 1986 Apr;89(4):530-2. doi: 10.1378/chest.89.4.530.
A limiting dilution assay was used to determine the frequency of T-lymphocytes reactive to purified protein derivative of tuberculin (PPD). Pleural fluid from patients with tuberculous pleurisy showed higher frequencies of PPD-reactive T-lymphocytes than peripheral blood from the same patients or tuberculin-positive healthy control subjects. The mean frequencies were 1/2,204 T cells in pleural fluid from tuberculous pleurisy, 1/14,970 T cells in peripheral blood from the same patients, and 1/13,130 T cells in peripheral blood from healthy controls. The concentration of tuberculin-reactive lymphocytes in tuberculous pleural fluid could represent selective accumulation or in situ expansion of this population of cell.
采用有限稀释分析法来确定对结核菌素纯蛋白衍生物（PPD）产生反应的T淋巴细胞的频率。结核性胸膜炎患者的胸腔积液中，PPD反应性T淋巴细胞的频率高于同一患者的外周血或结核菌素阳性健康对照者的外周血。平均频率分别为：结核性胸膜炎患者胸腔积液中每2,204个T细胞中有1个反应性T细胞，同一患者外周血中每14,970个T细胞中有1个，健康对照者外周血中每13,130个T细胞中有1个。结核性胸腔积液中结核菌素反应性淋巴细胞的浓度可能代表了该细胞群体的选择性积聚或原位扩增。
