Rozenszajn L A, Muellenberg-Coulombre C, Gery I, el-Saied M, Kuwabara T, Mochizuki M, Lando Z, Nussenblatt R B
Immunology. 1986 Apr;57(4):559-65.
Experimental autoimmune uveoretinitis was induced in genetically susceptible Lewis rats by passive transfer of T-lymphocyte cell lines from long-term cultures primed against soluble retinal antigen (S-Ag). A continuous T-cell line was established from non-adherent lymph node cells of S-Ag-immunized Lewis rats. The lymphoid cells were propagated in vitro by serially restimulating them with S-Ag in the presence of irradiated syngeneic spleen cells and expanding them in IL-2-containing media. The cell lines exhibited markers specific for T lymphocytes and the majority had the helper phenotype. When naïve rats were inoculated intravenously with anti S-Ag T-cell lines re-exposed to the antigen prior to injection, they developed uveoretinitis with both clinical and histological characteristics in half the time required by S-Ag to induce the disease by active immunization. The rats exhibited a delayed hypersensitivity skin reaction towards S-Ag.
通过被动转移来自长期培养且经可溶性视网膜抗原（S-Ag）致敏的T淋巴细胞系，在基因易感的Lewis大鼠中诱导实验性自身免疫性葡萄膜视网膜炎。从经S-Ag免疫的Lewis大鼠的非贴壁淋巴结细胞建立了一个连续的T细胞系。通过在经照射的同基因脾细胞存在的情况下用S-Ag连续再刺激淋巴细胞并在含白细胞介素-2的培养基中使其扩增，从而在体外培养这些淋巴细胞。这些细胞系表现出T淋巴细胞特异性标志物，并且大多数具有辅助性表型。当在注射前将重新暴露于抗原的抗S-Ag T细胞系静脉内接种给未致敏大鼠时，它们在S-Ag通过主动免疫诱导疾病所需时间的一半时间内就出现了具有临床和组织学特征的葡萄膜视网膜炎。这些大鼠对S-Ag表现出迟发型超敏皮肤反应。
