Chongqing Key Laboratory of Natural Product Synthesis and Drug Research, School of Pharmaceutical Sciences, Chongqing University, 55 Daxuecheng South Road, 401331, Shapingba, Chongqing, PR China.
Chongqing Key Laboratory of Natural Product Synthesis and Drug Research, School of Pharmaceutical Sciences, Chongqing University, 55 Daxuecheng South Road, 401331, Shapingba, Chongqing, PR China; Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences, Chongqing, 400714, PR China.
Anal Chim Acta. 2022 Jan 15;1190:339175. doi: 10.1016/j.aca.2021.339175. Epub 2021 Oct 15.
In this work, we developed a new approach for fabricating hollow and porous nitrogen doped carbon nanoballoons loading AuNPs (Au-NC-NBs) with a large specific surface area, a high N and Au content. The surface-enhanced Raman scattering (SERS) aptasensor based on the resulting Au-NC-NBs possess a wider linear range (10 to 10 cells/mL), a lower detection limit (3 cells/mL), better selectivity for detecting bacteria than previously reported sensors. Importantly, Au-NC-NBs SERS aptasensor also exhibits excellent performance for detecting bacteria in the real food and biological samples. This work provides a facile and versatile designing strategy for controlled construction of SERS biosensor by combination of Au nanoparticles and carbon materials, which has a great applied potential in food safety monitoring and clinical diagnosis.
在这项工作中，我们开发了一种新的方法来制造具有大比表面积、高氮和金含量的中空多孔氮掺杂碳纳米气球负载金纳米颗粒（Au-NC-NBs）。基于所得 Au-NC-NBs 的表面增强拉曼散射（SERS）适体传感器具有更宽的线性范围（10 到 10 个细胞/mL）、更低的检测限（3 个细胞/mL），比以前报道的传感器对检测细菌具有更好的选择性。重要的是，Au-NC-NBs SERS 适体传感器在检测实际食品和生物样本中的细菌时也表现出优异的性能。这项工作为通过金纳米颗粒和碳材料的组合来控制构建 SERS 生物传感器提供了一种简单而通用的设计策略，在食品安全监测和临床诊断方面具有巨大的应用潜力。
