Nik Kamarudin Nik Abdul Aziz, Mawang Christina Injan, Ahamad Mariana
Acarology Unit, Infectious Disease Research Center, Institute for Medical Research, National Institutes of Health, Ministry of Health Malaysia, Setia Alam 40170, Malaysia.
Biomedicines. 2023 Oct 18;11(10):2818. doi: 10.3390/biomedicines11102818.
sensu lato ( s.l.), which is predominantly spread by ticks, is the cause of Lyme disease (LD), also known as Lyme borreliosis, one of the zoonotic diseases affecting people. In recent years, LD has become more prevalent worldwide, even in countries with no prior records. Currently, Lyme Borrelia detection is achieved through nucleic acid amplification, antigen detection, microscopy, and in vitro culture. Nevertheless, these methods lack sensitivity in the early phase of the disease and, thus, are unable to confirm active infection. This review briefly discusses the existing direct detection methods of LD. Furthermore, this review also introduces the use of aptamer technology integrated with biosensor platforms to detect the Borrelia antigen. This aptamer technology could be explored using other biosensor platforms targeting whole Borrelia cells or specific molecules to enhance Borrelia detection in the future.
广义的（s.l.）莱姆病，主要通过蜱虫传播，是莱姆病（LD）的病因，莱姆病也被称为莱姆疏螺旋体病，是一种影响人类的人畜共患病。近年来，莱姆病在全球范围内变得更加普遍，甚至在以前没有记录的国家也是如此。目前，莱姆疏螺旋体的检测是通过核酸扩增、抗原检测、显微镜检查和体外培养来实现的。然而，这些方法在疾病的早期阶段缺乏敏感性，因此无法确认是否存在活动性感染。本文简要讨论了现有的莱姆病直接检测方法。此外，本文还介绍了将适体技术与生物传感器平台相结合用于检测疏螺旋体抗原的方法。未来，可以利用针对整个疏螺旋体细胞或特定分子的其他生物传感器平台来探索这种适体技术，以提高疏螺旋体的检测能力。
