Institute for Agricultural Product Quality Safety and Testing Technology, Guangxi Academy of Agricultural Sciences, Nanning, 530007, China.
Rice Research Institute, Guangxi Academy of Agricultural Sciences/Guangxi Key Laboratory of Rice Genetics and Breeding, Nanning, 530007, China.
Chemosphere. 2023 Nov;341:140033. doi: 10.1016/j.chemosphere.2023.140033. Epub 2023 Aug 31.
In this study, a novel electrochemical aptasensor for carbofuran (CBF) detection is prepared by gold nanoparticles decorated hierarchical porous carbon (Au@HPC). The prepared carbon materials show a three-dimensional hierarchical structure with a large specific surface area and a highly developed porous structure. Aptamers loading significantly improves when gold nanoparticles are embedded into the hierarchical porous carbon skeleton. Besides, Au@HPC modified electrode exhibits a large electroactive area and excellent electrochemical conductivity, serving as a promising platform for highly sensitive and selective electrochemical detection of CBF. The developed CBF electrochemical aptasensor shows a wide linear from 1.0 to 100000 pg/L with a detection limit of 0.5 pg/L, demonstrating an extraordinary sensitivity compared to other sensors for CBF detection. Additionally, the designed aptasensor was used to monitor the CBF in vegetable samples, with a recovery range from 98.4% to 104.8%. The results coincide with the standard test method, revealing its practicability in the food safety analysis.
在这项研究中，通过金纳米粒子修饰的分级多孔碳（Au@HPC）制备了用于检测克百威（CBF）的新型电化学适体传感器。所制备的碳材料具有三维分级结构，具有大的比表面积和高度发达的多孔结构。当金纳米粒子嵌入分级多孔碳骨架中时，载量明显提高。此外，Au@HPC 修饰电极具有大的电活性面积和优异的电化学导电性，可用作高灵敏度和选择性电化学检测 CBF 的有前途的平台。所开发的 CBF 电化学适体传感器具有从 1.0 到 100000 pg/L 的宽线性范围和 0.5 pg/L 的检测限，与其他用于 CBF 检测的传感器相比具有非凡的灵敏度。此外，该设计的适体传感器用于监测蔬菜样品中的 CBF，回收率范围为 98.4%至 104.8%。结果与标准测试方法吻合，表明其在食品安全分析中的实用性。
