Department of Prosthodontics, Guanghua School of Stomatology, Hospital of Stomatology, Guangdong Provincial Key Laboratory of Stomatology, Sun Yat-sen University, Guangzhou, Guangdong, PR China.
Department of Orthodontics, Guanghua School of Stomatology, Hospital of Stomatology, Guangdong Provincial Key Laboratory of Stomatology, Sun Yat-sen University, Guangzhou, Guangdong, PR China.
Mater Sci Eng C Mater Biol Appl. 2021 Dec;131:112473. doi: 10.1016/j.msec.2021.112473. Epub 2021 Oct 13.
Environments with high reactive oxygen species (ROS) levels, which are common in patients with diseases such as diabetes, periodontitis, and osteoporosis, impair the osseointegration of implants. To address this issue, by using a one-pot dopamine-assisted co-deposition method, we constructed a three-dimensional coating of hydroxyapatite-functionalised nanoparticles of polydopamine (HA/nPDAs) on implant surfaces, where polydopamine is designed to protect cells via scavenging excessive ROS and HA facilitates osteogenesis. First, nanoparticles of polydopamine (nPDAs) were prepared by self-polymerization and assembly of dopamine under alkaline conditions, and HA/nPDAs were obtained by incubating nPDAs in simulated body fluid (SBF) due to metal chelation and ionic interactions triggered by the catechol moieties of PDA. Thereafter, HA/nPDAs with thickness of ~4 μm were constructed on titanium surfaces by immersing titanium discs in a weak alkaline solution of HA/nPDAs and dopamine through interface interactions driven by catechol chemistry. The properties of coatings (e.g., thickness, composition, hydrophilia and morphology) can be controlled by preparation conditions such as mineralization time and reactant concentration. The coatings display efficient ROS-scavenging ability, promote cell proliferation, and upregulate the activity of alkaline phosphatase and the expression of osteogenesis-related genes in environments with high or normal ROS levels, demonstrating the great promise of such coatings for osseointegration promotion, especially in the state of high ROS in diseases. This study provides a facile, efficient, mild, and universal strategy in engineering functional surfaces on any substrates for diversified applications by simple variation of co-deposited components, through taking advantages of versatile catechol chemistry and nanoparticles with stereo structure and great specific surface area.
在患有糖尿病、牙周炎和骨质疏松症等疾病的患者中，环境中活性氧（ROS）水平较高，会损害植入物的骨整合。为了解决这个问题，我们通过使用多巴胺辅助的一锅共沉积方法，在植入物表面构建了一层具有纳米羟基磷灰石功能化的聚多巴胺（HA/nPDAs）的三维涂层，其中聚多巴胺通过清除过多的 ROS 来保护细胞，而 HA 则促进成骨。首先，通过在碱性条件下多巴胺的自聚合和组装制备纳米聚多巴胺（nPDAs），然后通过将 nPDAs 孵育在模拟体液（SBF）中，由于 PDA 的儿茶酚部分引发的金属螯合和离子相互作用，获得 HA/nPDAs。此后，通过将钛盘浸入 HA/nPDAs 和多巴胺的弱碱性溶液中，利用儿茶酚化学驱动的界面相互作用，在钛表面构建厚度约为 4μm 的 HA/nPDAs 涂层。涂层的性质（例如厚度、组成、亲水性和形态）可以通过矿化时间和反应物浓度等制备条件来控制。涂层具有高效的 ROS 清除能力，可促进细胞增殖，并在高 ROS 或正常 ROS 水平的环境中上调碱性磷酸酶的活性和骨形成相关基因的表达，这表明这种涂层在促进骨整合方面具有很大的潜力，特别是在疾病状态下 ROS 较高的情况下。这项研究提供了一种简便、高效、温和且通用的策略，通过简单改变共沉积成分，利用多功能儿茶酚化学和具有立体结构和大比表面积的纳米粒子，在任何基底上工程功能表面，用于多样化的应用。
