School of Materials Science and Engineering, South China University of Technology, Guangzhou 510641, China; National Engineering Research Center for Tissue Restoration and Reconstruction, Guangzhou 510006, China.
National Engineering Research Center for Tissue Restoration and Reconstruction, Guangzhou 510006, China; Medprin Regenerative Medical Technologies Co., Ltd, Guangzhou 510663, China.
Mater Sci Eng C Mater Biol Appl. 2021 Dec;131:112490. doi: 10.1016/j.msec.2021.112490. Epub 2021 Oct 14.
Calcium phosphate cement (CPC) with good injectability and osteoconductivity plays important roles in bone grafting application. Much attention has been paid to achieve multifunctionality through incorporating trace elements into CPC. Silicon and zinc can be used as additives to endow CPC with biological functions of osteogenesis, angiogenesis and anti-osteoclastogenesis. In this study, zinc and silicate ions were co-incorporated into CPC through mixing with submicron zinc silicate (ZnSiO, ZS) to obtain zinc silicate-modified CPCs (ZS/CPCs) with different contents. The results revealed that the addition of ZS increased the compressive strength, prolonged the setting time, and densified the structure of CPC. Low addition content of ZS facilitated the formation of surface apatite layer in the early mineralization stage. Incorporating ZS significantly induced osteogenesis of mouse bone marrow stromal cells (mBMSCs) and angiogenesis of human umbilical vein endothelial cells (HUVECs), and moreover, restricted osteoclastogenesis of Raw 264.7 in vitro. Silicate and zinc ions could be steadily released from ZS/CPCs into the culture medium. With the synergistic effect of silicate and zinc ions, ZS/CPCs provided an appropriate microenvironment for the immune cells to facilitate the osteogenesis of mBMSCs and angiogenesis of HUVECs further. Taken together, it can be concluded that incorporating ZS is an effective way to endow CPC with multifunctionality and better bone regeneration for bone defect repair.
具有良好可注射性和骨传导性的磷酸钙水泥 (CPC) 在骨移植应用中起着重要作用。通过将微量元素掺入 CPC 中以实现多功能性引起了广泛关注。硅和锌可用作添加剂，赋予 CPC 成骨、血管生成和抗破骨细胞生成的生物学功能。在这项研究中，通过与亚微米硅锌矿 (ZnSiO，ZS) 混合，将锌和硅酸盐离子共掺入 CPC 中，获得了具有不同含量的硅锌酸盐修饰的 CPC（ZS/CPC）。结果表明，ZS 的添加提高了 CPC 的抗压强度、延长了凝固时间并使 CPC 的结构更加致密。ZS 的低添加含量促进了早期矿化阶段表面磷灰石层的形成。ZS 的掺入显著诱导了小鼠骨髓基质细胞 (mBMSCs) 的成骨作用和人脐静脉内皮细胞 (HUVECs) 的血管生成作用，并且体外还限制了 Raw 264.7 的破骨细胞生成。硅酸盐和锌离子可以从 ZS/CPC 中稳定地释放到培养基中。硅酸盐和锌离子的协同作用为免疫细胞提供了适当的微环境，进一步促进 mBMSCs 的成骨作用和 HUVECs 的血管生成作用。综上所述，可以得出结论，掺入 ZS 是赋予 CPC 多功能性和更好的骨再生以修复骨缺损的有效方法。
