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具有免疫调节作用的含锌壳聚糖/明胶涂层用于牙种植体周围软组织封闭的研发

Development of Zinc-Containing Chitosan/Gelatin Coatings with Immunomodulatory Effect for Soft Tissue Sealing around Dental Implants.

作者信息

Han Jing, Sanders Jorine G F, Andrée Lea, van Oirschot Bart A J A, Plachokova Adelina S, van den Beucken Jeroen J J P, Leeuwenburgh Sander C G, Yang Fang

机构信息

Department of Dentistry-Regenerative Biomaterials, Research Institute for Medical Innovation, Radboudumc, Philips Van Leijdenlaan 25, 6525, Nijmegen, The Netherlands.

出版信息

Tissue Eng Regen Med. 2025 Jan;22(1):57-75. doi: 10.1007/s13770-024-00680-y. Epub 2024 Nov 23.

DOI:10.1007/s13770-024-00680-y
PMID:39579168
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11711843/
Abstract

BACKGROUND

Soft tissue integration (STI) around dental implant abutments is a prerequisite to prevent bacterial invasion and achieve successful dental implant rehabilitation. However, peri-implant STI is a major challenge after dental abutment placement due to alterations in the immune microenvironment upon surgical dental implant installation.

METHODS

Based on known immunomodulatory effects of zinc, we herein deposited zinc/chitosan/gelatin (Zn/CS/Gel) coatings onto titanium substrates to study their effect on macrophages. First, we exposed macrophages to cell culture media containing different zinc ion (Zn) concentrations. Next, we explored the immunomodulatory effect of Zn/CS/Gel coatings prepared via facile electrophoretic deposition (EPD).

RESULTS

We found that Zn effectively altered the secretome by reducing the secretion of pro-inflammatory and enhancing pro-regenerative cytokine secretion, particularly at a Zn supplementation of approximately 37.5 μM. Zn/CS/Gel coatings released Zn in a concentration range which effectively stimulated pro-regenerative macrophage polarization as demonstrated by M2 macrophage polarization. Additionally, the impact of these Zn-exposed macrophages on gingival fibroblasts incubated in conditioned medium showed stimulated adhesion, proliferation, and collagen secretion.

CONCLUSION

Our promising results suggest that controlled release of Zn from Zn/CS/Gel coatings could be applied to immunomodulate peri-implant STI, and to enhance dental implant survival.

摘要

背景

牙种植体基台周围的软组织整合（STI）是预防细菌入侵并实现成功牙种植修复的前提条件。然而，由于种植手术时免疫微环境的改变，种植体基台植入后的种植体周围软组织整合是一项重大挑战。

方法

基于锌已知的免疫调节作用，我们在此将锌/壳聚糖/明胶（Zn/CS/Gel）涂层沉积在钛基底上，以研究其对巨噬细胞的影响。首先，我们将巨噬细胞暴露于含有不同锌离子（Zn）浓度的细胞培养基中。接下来，我们探究了通过简便的电泳沉积（EPD）制备的Zn/CS/Gel涂层的免疫调节作用。

结果

我们发现，锌通过减少促炎细胞因子的分泌并增强促再生细胞因子的分泌，有效地改变了分泌组，特别是在锌补充量约为37.5 μM时。Zn/CS/Gel涂层释放的锌浓度范围有效地刺激了促再生巨噬细胞极化，如M2巨噬细胞极化所示。此外，这些暴露于锌的巨噬细胞对在条件培养基中培养的牙龈成纤维细胞的影响表现为刺激其黏附、增殖和胶原蛋白分泌。

结论

我们的研究结果表明，从Zn/CS/Gel涂层中可控释放锌可用于免疫调节种植体周围软组织整合，并提高牙种植体的存活率。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8782/11711843/bb31a98c8c32/13770_2024_680_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8782/11711843/c214d3ea4f66/13770_2024_680_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8782/11711843/1439f372aabc/13770_2024_680_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8782/11711843/5fed92978a96/13770_2024_680_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8782/11711843/71e32ece3d06/13770_2024_680_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8782/11711843/2d279feb2c48/13770_2024_680_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8782/11711843/869cc005d8bb/13770_2024_680_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8782/11711843/91c532b70c3c/13770_2024_680_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8782/11711843/bb31a98c8c32/13770_2024_680_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8782/11711843/c214d3ea4f66/13770_2024_680_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8782/11711843/1439f372aabc/13770_2024_680_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8782/11711843/5fed92978a96/13770_2024_680_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8782/11711843/71e32ece3d06/13770_2024_680_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8782/11711843/2d279feb2c48/13770_2024_680_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8782/11711843/869cc005d8bb/13770_2024_680_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8782/11711843/91c532b70c3c/13770_2024_680_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8782/11711843/bb31a98c8c32/13770_2024_680_Fig8_HTML.jpg

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