Lee Sun Hwa, Cai Mudan, Yang Eun Jin
Department of Clinical Research, Korea Institute of Oriental Medicine, Daejeon, South Korea.
Department of Korea Medicine (KM) Science Research, Korea Institute of Oriental Medicine, Daejeon, South Korea.
Front Neurosci. 2021 Nov 11;15:743705. doi: 10.3389/fnins.2021.743705. eCollection 2021.
Amyotrophic lateral sclerosis (ALS) is a complex disease characterized by motor neuron loss and muscle atrophy. There is no prominent treatment for ALS as the pathogenic process in the skeletal muscle and spinal cord is complex and multifactorial. Therefore, we investigated the effects of a herbal formula on the multi-target effects in the skeletal muscle and spinal cord in hSOD1 transgenic mice. We prepared a herbal extract (HE) from , , , and . Control and HE-treated mice underwent rotarod and footprint tests. We also performed immunohistochemical and Western blotting analyses to assess expression of inflammation-related and oxidative stress-related proteins in the muscle and spinal cord tissues. We found that the HE increased motor activity and reduced motor neuron loss in hSOD1 mice. In addition, the HE significantly reduced the levels of inflammatory proteins and oxidative stress-related proteins in the skeletal muscles and spinal cord of hSOD1 mice. Furthermore, we demonstrated that the HE regulated autophagy function and augmented neuromuscular junction in the muscle of hSOD1 mice. Based on these results, we propose that the HE formula may be a potential therapeutic strategy for multi-target treatment in complex and multifactorial pathological diseases.
肌萎缩侧索硬化症（ALS）是一种以运动神经元丧失和肌肉萎缩为特征的复杂疾病。由于骨骼肌和脊髓中的致病过程复杂且多因素，目前尚无针对ALS的有效治疗方法。因此，我们研究了一种中药配方对hSOD1转基因小鼠骨骼肌和脊髓多靶点效应的影响。我们从[具体药材名称1]、[具体药材名称2]、[具体药材名称3]和[具体药材名称4]制备了一种草药提取物（HE）。对对照组和接受HE治疗的小鼠进行了转棒试验和足迹试验。我们还进行了免疫组织化学和蛋白质印迹分析，以评估肌肉和脊髓组织中炎症相关蛋白和氧化应激相关蛋白的表达。我们发现，HE增加了hSOD1小鼠的运动活性并减少了运动神经元丧失。此外，HE显著降低了hSOD1小鼠骨骼肌和脊髓中炎症蛋白和氧化应激相关蛋白的水平。此外，我们证明了HE调节了hSOD1小鼠肌肉中的自噬功能并增强了神经肌肉接头。基于这些结果，我们提出HE配方可能是一种针对复杂多因素病理疾病进行多靶点治疗的潜在策略。
