Department of Orthopaedics, Peking University Third Hospital, Beijing, China.
Peking University Third Hospital, Beijing Key Laboratory of Spinal Disease Research, Beijing, China.
Front Endocrinol (Lausanne). 2021 Nov 9;12:763538. doi: 10.3389/fendo.2021.763538. eCollection 2021.
This study aimed to explore the association between serum amino acids (AAs) levels and bone mineral density (BMD).
We performed a two-sample Mendelian randomization (MR) analysis to analyze the associations between the levels of eight AAs and BMD values by using summary-level genome-wide association study (GWAS) data. We applied the MR Steiger filtering method and MR Pleiotropy RESidual Sum and Outlier (MR-PRESSO) global test to check for and remove single nucleotide polymorphisms (SNPs) that were horizontally pleiotropic. The associations were estimated with the inverse variance weighted (IVW), MR-Egger, weighted median and MR Robust Adjusted Profile Score (MR.RAPS) methods.
Our study found that genetically increased isoleucine (Ile) [IVW: effect = 0.1601, 95% confidence interval (CI) = 0.0604 ~ 0.2597, p = 0.0016] and valine (Val) levels (IVW: effect = 0.0953, 95% CI = 0.0251 ~ 0.1655, p = 0.0078) were positively associated with total body BMD (TB-BMD). The results also revealed that genetically increased tyrosine (Tyr) levels were negatively associated with TB-BMD (IVW: effect = -0.1091, 95% CI = -0.1863 ~ -0.0320, p = 0.0055).
In this study, associations between serum AA levels and BMD were established. These findings underscore the important role that serum AAs play in the development of osteoporosis and provide evidence that osteoporosis can be prevented and treated by the intake of certain AAs.
本研究旨在探讨血清氨基酸（AA）水平与骨密度（BMD）之间的关系。
我们进行了两样本孟德尔随机化（MR）分析，使用汇总水平全基因组关联研究（GWAS）数据来分析八种 AA 水平与 BMD 值之间的关联。我们应用 MR Steiger 过滤法和 MR Pleiotropy RESidual Sum and Outlier（MR-PRESSO）全局检验来筛选和去除水平遗传关联的单核苷酸多态性（SNP）。使用逆方差加权（IVW）、MR-Egger、加权中位数和 MR 稳健调整轮廓得分（MR.RAPS）方法估计关联。
我们的研究发现，遗传上升高的异亮氨酸（Ile）[IVW：效应=0.1601，95%置信区间（CI）=0.06040.2597，p=0.0016]和缬氨酸（Val）水平（IVW：效应=0.0953，95%CI=0.02510.1655，p=0.0078）与全身骨密度（TB-BMD）呈正相关。结果还表明，遗传上升高的酪氨酸（Tyr）水平与 TB-BMD 呈负相关（IVW：效应=-0.1091，95%CI=-0.1863~-0.0320，p=0.0055）。
本研究建立了血清 AA 水平与 BMD 之间的关联。这些发现强调了血清 AA 在骨质疏松症发展中的重要作用，并为通过摄入某些 AA 来预防和治疗骨质疏松症提供了证据。
