Jiang Xinyan, Pu Rui, Wang Cheng, Xu Jiale, Tang Yaohui, Qi Shuhong, Zhan Qiuqiang, Wei Xunbin, Gu Bobo
Med-X Research Institute and School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai 200030, China.
These authors contributed equally.
Biomed Opt Express. 2021 Oct 18;12(11):6984-6994. doi: 10.1364/BOE.442657. eCollection 2021 Nov 1.
Acquired brain injury (ABI), which is the umbrella term for all brain injuries, is one of the most dangerous diseases resulting in high morbidity and mortality, making it extremely significant to early diagnosis of ABI. Current methods, which are mainly composed of X-ray computed tomography and magnetic resonance angiography, remain limited in diagnosis of ABI with respect to limited spatial resolution and long scanning times. Here, we reported through-skull fluorescence imaging of mouse cerebral vasculature without craniotomy, utilizing the fluorescence of down-conversion nanoparticles (DCNPs) in the 1.3 - 1.7 μm near-infrared window (NIR-II window). Due to its high spatial resolution of 22.79 μm, the NIR-II fluorescence imaging method could quickly distinguish the brain injury region of mice after performing the stab wound injury (traumatic brain injury) and ischemic stroke (non-traumatic brain injury), enabling it a powerful tool in the noninvasive and early diagnosis of ABI.
获得性脑损伤（ABI）是所有脑损伤的统称，是导致高发病率和死亡率的最危险疾病之一，因此对ABI进行早期诊断极为重要。目前的方法主要由X射线计算机断层扫描和磁共振血管造影组成，在诊断ABI方面，由于空间分辨率有限和扫描时间长，仍然存在局限性。在此，我们报道了无需开颅的小鼠脑血管系统穿颅荧光成像，利用了1.3 - 1.7μm近红外窗口（NIR-II窗口）中的下转换纳米粒子（DCNPs）的荧光。由于其22.79μm的高空间分辨率，NIR-II荧光成像方法在对小鼠进行刺伤损伤（创伤性脑损伤）和缺血性中风（非创伤性脑损伤）后能够快速区分脑损伤区域，使其成为ABI无创和早期诊断的有力工具。
