Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, College of Optoelectronic Engineering , Shenzhen University , Shenzhen 518060 , China.
Molecular Imaging Program at Stanford (MIPS), Bio-X Program, and Department of Radiology, Canary Center at Stanford for Cancer Early Detection , Stanford University , Stanford , California 94305-5344 , United States.
Nano Lett. 2019 May 8;19(5):2985-2992. doi: 10.1021/acs.nanolett.9b00140. Epub 2019 Apr 17.
As a newly noninvasive emerging modality, NIR-II fluorescence imaging (1000-1700 nm) has many advantages over conventional visible and NIR-I imaging (700-900 nm). Unfortunately, only a few NIR-II fluorophores are suitable for bone imaging. Here, we report an NIR-II fluorophore based on DSPE-mPEG encapsulated rare earth doped nanoparticles (RENPs@DSPE-mPEG), which shows inherent affinity to bone without linking any targeting ligands, and thus, it provides an alternative noninvasive and nonradiation strategy for skeletal system mapping and bone disease diagnoses. Interestingly, within the NIR-II window, imaging at a longer wavelength (1345 nm) provides a higher resolution and signal-to-noise ratio than imaging at 1064 nm, even though the quantum yield at 1064 nm is 2-fold higher than that at 1345 nm. Besides bone imaging, RENPs@DSPE-mPEG show an imaging application in blood vessels and lymph nodes. Importantly, RENPs@DSPE-mPEG can be internalized by circulating white blood cells. This finding may open a window to increase efficient nanoparticle delivery in the fields such as immunotherapy and improve the diagnostic and therapeutic efficacy of cancer-targeted nanoparticles in clinical applications.
作为一种新兴的无创模态，近红外二区（NIR-II）荧光成像是一种比传统的可见光和近红外一区（NIR-I）成像更有优势的技术（1000-1700nm）。不幸的是，只有少数近红外二区荧光团适用于骨成像。在这里，我们报告了一种基于 DSPE-mPEG 包裹的稀土掺杂纳米颗粒（RENPs@DSPE-mPEG）的近红外二区荧光团，它具有与骨骼的固有亲和力，而不需要连接任何靶向配体，因此，它为骨骼系统成像和骨病诊断提供了一种替代的无创和非辐射策略。有趣的是，在近红外二区窗口内，在较长波长（1345nm）进行成像比在 1064nm 处进行成像具有更高的分辨率和信噪比，尽管在 1064nm 处的量子产率是在 1345nm 处的两倍。除了骨成像外，RENPs@DSPE-mPEG 还在血管和淋巴结成像方面具有应用。重要的是，RENPs@DSPE-mPEG 可以被循环白细胞内化。这一发现可能为提高免疫治疗等领域的有效纳米颗粒递送打开一扇窗，并提高癌症靶向纳米颗粒在临床应用中的诊断和治疗效果。
