School of Biomedical Engineering, Shanghai Jiao Tong University, 1954 Huashan Road, Shanghai, 200030, China.
State Key Laboratory of Organic-Inorganic Composites, Beijing University of Chemical Technology, Beijing, 100029, China.
Anal Chim Acta. 2021 Apr 15;1154:338309. doi: 10.1016/j.aca.2021.338309. Epub 2021 Feb 10.
Cancer severely threatens human health currently, promoting the rapid development of cancer treatment strategies. In addition to cancer therapy, assessment of cancer prognosis, which can evaluate the success with treatment and chances of recovery as well as assist to make subsequent therapeutic schedule, is also remarkably indispensable and important. Conventional technologies can't provide rapid and highly-sensitive assessment of cancer prognosis at cytological level. Herein, an effective nitrogen doped carbon dots with intrinsic nucleolus-targeting capability and high fluorescence quantum yield are synthesized, characterized and employed for fluorescence imaging of nucleolus, which is closely related to the biological alteration of cancer cell. The cancer prognosis thus can be accurately (limit of detection: 50 nM) and rapidly (5 min) assessed at subcellular organelle level from nucleolar characteristics, which are visualized and analyzed by the captured fluorescence images. Outstanding assessment performance endows the proposed technology with great potential for future clinical research.
目前，癌症严重威胁着人类健康，促使癌症治疗策略快速发展。除了癌症治疗外，癌症预后评估也非常不可或缺和重要，它可以评估治疗的成功率和康复机会，并有助于制定后续的治疗计划。传统技术无法在细胞学水平上提供对癌症预后的快速和高灵敏度评估。在此，合成了一种具有内在核仁靶向能力和高光致荧光量子产率的有效氮掺杂碳点，并对其进行了表征，用于核仁的荧光成像，这与癌细胞的生物学变化密切相关。通过捕获的荧光图像，从核仁特征上可以在亚细胞器水平上快速（5 分钟）和准确（检测限：50 nM）地对癌症预后进行评估。出色的评估性能使该技术具有巨大的临床研究潜力。
