2 型糖尿病对小鼠下颌骨再生及辅助性 T 细胞 17/调节性 T 细胞相关因子表达的影响。
Effect of type 2 diabetes mellitus on mandibular bone regeneration and the expression of T helper cell 17/regulat-ory T cell-related factors in mice.
机构信息
Dept. of Implantology, School and Hospital of Stomatology, Cheeloo College of Medicine, Shandong University & Shandong Key Laboratory of Oral Tissue Regeneration & Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration, Jinan 250012, China.
Dept. of Implantology, Yuehu Branch, Ningbo Dental Hospital, Ningbo 315000, China.
出版信息
Hua Xi Kou Qiang Yi Xue Za Zhi. 2021 Dec 1;39(6):642-650. doi: 10.7518/hxkq.2021.06.004.
OBJECTIVES
To observe the effect of type 2 diabetes mellitus (T2DM) on mandibular bone regeneration and the expression of factors related to T helper cell 17 (Th17 cell) and regulatory T cell (Treg cell) in mice.
METHODS
Thirty-six 6-week-old C57BL/6J male mice were randomly divided into normal control (NC) and T2DM groups. Fasting blood glucose levels were detected 0 d, 7 d, 14 d, and 28 d after surgery for mandibular defects. Hematoxylin-eosin (HE) staining was used in observing the bone after 7 d, 14 d, and 28 d of the healing process. Immunohistochemical staining was used in observing the expression of alkaline phosphatase (ALP), Runt-related transcription factor 2 (RUNX2), forkhead box protein P3 (Foxp3), retinoic acid related orphan receptor gamma T (RORγt), and protein tyrosine phosphatase non-receptor type 2 (PTPN2) after 7 d, 14 d, and 28 d of healing.
RESULTS
HE staining showed that the area with new bones in the T2DM group was significantly smaller than that in the NC group. Immunohistochemical staining showed that the expression of osteogenesis related proteins ALP and RUNX2 were significantly reduced in the T2DM group. In addition, the number of RORγt positive cells increased, whereas the number of Foxp3 positive cells and the expression PTPN2 decreased significantly in the mandibular bone defect in mice with T2DM.
CONCLUSIONS
T2DM significantly inhibit mandibular bone regeneration in mice. Decline in PTPN2 expression and the transition of Treg and Th17 may be the underlying molecular mechanisms.
目的
观察 2 型糖尿病（T2DM）对小鼠下颌骨再生的影响及与辅助性 T 细胞 17（Th17 细胞）和调节性 T 细胞（Treg 细胞）相关因子的表达。
方法
36 只 6 周龄 C57BL/6J 雄性小鼠随机分为正常对照组（NC 组）和 T2DM 组。术后 0、7、14、28 d 检测空腹血糖水平，7、14、28 d 检测下颌骨缺损愈合过程中骨的苏木精-伊红（HE）染色。免疫组织化学染色观察碱性磷酸酶（ALP）、 runt 相关转录因子 2（RUNX2）、叉头框蛋白 P3（Foxp3）、维甲酸相关孤儿受体γ T（RORγt）和蛋白酪氨酸磷酸酶非受体型 2（PTPN2）在愈合后 7、14、28 d 的表达。
结果
HE 染色显示 T2DM 组新骨面积明显小于 NC 组。免疫组织化学染色显示 T2DM 组成骨相关蛋白 ALP 和 RUNX2 的表达明显降低。此外，T2DM 小鼠下颌骨骨缺损中 RORγt 阳性细胞数增加，而 Foxp3 阳性细胞数和 PTPN2 表达明显减少。
结论
T2DM 显著抑制小鼠下颌骨再生，下调 PTPN2 表达及 Treg 和 Th17 细胞的转化可能是其潜在的分子机制。
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