Department of Biomedical Engineering, National University of Singapore, 4 Engineering Drive 3, 04-08, 117583, Singapore.
Institute for Health Innovation and Technology (iHealthtech), MD6, 14 Medical Drive #14-01, Singapore 117599.
Nanoscale. 2021 Dec 16;13(48):20531-20540. doi: 10.1039/d1nr06594g.
Lymphocytes play a vital role in immunosurveillance through sensing biomolecules and eliminating targeted invaders. Compared with conventional therapies that depend on drug loading, lymphocytes are advantageous as they are able to ensure self-regulated therapeutics. Here, novel multi-compartmental DNA hydrogel particles were synthesized using a microfluidic assembly for intelligent cancer treatment the logic-based control of siRNA release without external stimulation. The sensing sequence (D1) was compartmentalized from the treatment sequence (D2) with the use of core-shell DNA hydrogel particles. When D1 detects a cancer-associated biomarker, miRNA-21, a sequence cascade is triggered to release siRNA from D2, effectively eliminating the targeted cancer cells lymphocyte-inspired precision medicine.
淋巴细胞在免疫监视中通过感知生物分子和清除靶向入侵者发挥着至关重要的作用。与依赖药物负载的传统疗法相比，淋巴细胞具有自我调节治疗的优势。在这里，使用微流控组装合成了新型多隔室 DNA 水凝胶颗粒，用于智能癌症治疗——无需外部刺激即可基于逻辑控制 siRNA 的释放。使用核壳 DNA 水凝胶颗粒将传感序列 (D1) 与治疗序列 (D2) 分隔开。当 D1 检测到与癌症相关的生物标志物 miRNA-21 时，会触发序列级联反应，从而从 D2 中释放 siRNA，有效地消除靶向癌细胞——受淋巴细胞启发的精准医学。
