Universiti Kebangsaan Malaysia (UKM), Institute of Microengineering and Nanoelectronics (IMEN), Bangi, Selangor, Malaysia.
Electrophoresis. 2022 Feb;43(4):609-620. doi: 10.1002/elps.202100207. Epub 2021 Dec 16.
This article describes a dielectrophoresis (DEP)-based simulation and experimental study of human epidermal keratinocyte (HEK) cells for wounded skin cell migration toward rapid epithelialization. MyDEP is a standalone software designed specifically to study dielectric particles and cell response to an alternating current (AC) electric field. This method demonstrated that negative dielectrophoresis (N ) occurs in HEK cells at a wide frequency range in highly conductive medium. The finite element method was used to characterize particle trajectory based on DEP and drag force. The performance of the system was assessed using HEK cells in a highly conductive EpiLife suspending medium. The DEP experiment was performed by applying sinusoidal wave AC potential at the peak-to-peak voltage of 10 V in a tapered aluminum microelectrode array from 100 kHz to 1 MHz. We experimentally observed the occurrence of NDEP, which attracted HEK cells toward the local electric field minima in the region of interest. The DIPP-MotionV software was used to track cell migration in the prerecorded video via an automatic marker and estimate the average speed and acceleration of the cells. The results showed that HEK cell migration was accomplished approximately at 6.43 μm/s at 100 kHz with 10 V, and F caused the cells to migrate and align at the target position, which resulted in faster wound closures because of the application of an electric field frequency to HEK cells in random locations.
本文描述了一种基于介电泳（DEP）的模拟和实验研究，用于研究人类表皮角质细胞（HEK）向受伤皮肤细胞迁移以实现快速上皮化的过程。MyDEP 是一款专为研究介电粒子和细胞对交流（AC）电场的响应而设计的独立软件。该方法表明，在高导电性介质中，HEK 细胞在很宽的频率范围内会发生负介电泳（NDEP）。有限元方法用于基于 DEP 和阻力对粒子轨迹进行特征化。使用高度导电的 EpiLife 悬浮介质中的 HEK 细胞来评估系统的性能。通过在从 100 kHz 到 1 MHz 的峰值到峰值电压为 10 V 的锥形铝微电极阵列中施加正弦波 AC 电势来进行 DEP 实验。我们实验观察到了 NDEP 的发生，它将 HEK 细胞吸引到感兴趣区域的局部电场最小值处。使用 DIPP-MotionV 软件通过自动标记跟踪预录制视频中的细胞迁移，并估计细胞的平均速度和加速度。结果表明，在 100 kHz 时，HEK 细胞的迁移速度约为 6.43 μm/s，施加 10 V 的电压，F 导致细胞迁移并在目标位置对齐，这使得由于在随机位置向 HEK 细胞施加电场频率，伤口闭合得更快。
