School of Agriculture and Biology, Shanghai Jiao Tong University, 800 Dongchuan Road, Minhang District, Shanghai, 200240, China.
Planta. 2021 Dec 3;255(1):12. doi: 10.1007/s00425-021-03698-x.
SmMYB35, a light-responsive R2R3-MYB transcription factor, positively regulates anthocyanin biosynthesis in eggplant by binding to the promoters of SmCHS, SmF3H, SmDFR, and SmANS and enhancing their activities. In addition, SmMYB35 interacts with SmTT8 and SmTTG1 to form a MBW complex, thereby enhancing anthocyanin biosynthesis. Eggplant is a vegetable rich in anthocyanins. SmMYB35, a light-responsive R2R3-MYB transcription factor, was isolated from eggplant and investigated for its biological functions. The results suggested that the expression of SmMYB35 was regulated by SmHY5 through directly binding to G-box in the promoter region, and the overexpression of SmMYB35 could increase the anthocyanin content in the stems and petals of the transgenic eggplants. SmMYB35 could also bind to the promoters of SmCHS, SmF3H, SmDFR, and SmANS and enhance their activities. In addition, SmMYB35 interacted with SmTT8 and SmTTG1 to form a MBW complex which enhanced anthocyanin biosynthesis. Taking together, we firstly verified that SmMYB35 promoted anthocyanin biosynthesis in plants. The results provide new insights into the regulatory effects of SmMYB35 on key anthocyanin biosynthetic genes and advance our understanding of the molecular mechanism of light-induced anthocyanin synthesis in eggplants.
SmMYB35，一种光响应的 R2R3-MYB 转录因子，通过结合 SmCHS、SmF3H、SmDFR 和 SmANS 的启动子并增强其活性，正向调控茄子中的花青素生物合成。此外，SmMYB35 与 SmTT8 和 SmTTG1 相互作用形成 MBW 复合物，从而增强花青素生物合成。茄子是一种富含花青素的蔬菜。从茄子中分离出光响应的 R2R3-MYB 转录因子 SmMYB35，并研究其生物学功能。结果表明，SmHY5 通过直接结合启动子区域的 G 盒来调节 SmMYB35 的表达，过表达 SmMYB35 可以增加转基因茄子茎和花瓣中的花青素含量。SmMYB35 还可以结合 SmCHS、SmF3H、SmDFR 和 SmANS 的启动子并增强其活性。此外，SmMYB35 与 SmTT8 和 SmTTG1 相互作用形成 MBW 复合物，从而增强花青素生物合成。总之，我们首次验证了 SmMYB35 促进了植物中的花青素生物合成。这些结果为 SmMYB35 对关键花青素生物合成基因的调控作用提供了新的见解，并深入了解了茄子中光诱导花青素合成的分子机制。
