Li Doudou, Chen Langjie, Cai Xinyan, Qi Yixiang, Lu Yongyue
Department of Entomology, College of Plant Protection, South China Agricultural University, Guangzhou 510642, China.
Insects. 2024 Jul 26;15(8):569. doi: 10.3390/insects15080569.
Diptera and Lepidoptera species have the highest levels of insecticide resistance, and the mechanism of drug resistance has been studied in detoxification metabolism genes such as P450, GST, EST, and ABC. Since are resistant to a variety of chemicals, the pattern and mechanism of resistance in have been investigated from a variety of aspects such as detoxification metabolism genes, detoxification enzymes, intestinal symbiotic bacteria, and synergists in the world. In this study, 51 species and 149 detoxification metabolism genes were annotated in the Suppression Subtractive Hybridization (SSH) library, and 12 candidate genes related to beta-cypermethrin resistance were screened and quantitatively expressed in this library. Two genes were found to be upregulated in the egg stage, three genes in the larval stage, one gene in the pupal stage, and five genes in the adult stage, and four genes were found to be upregulated in the midgut and the malacca ducts in the midgut. The expression of , , , and genes was upregulated in resistant strains, with the most obvious upregulation occurring in the midgut and the Malpighian tubules. These results provide new insights into the study of pesticide resistance in quarantine insects.
双翅目和鳞翅目昆虫具有最高水平的杀虫剂抗性，并且已经在诸如P450、GST、EST和ABC等解毒代谢基因中研究了抗药性机制。由于[此处原文缺失相关内容]对多种化学物质具有抗性，因此在世界范围内已经从解毒代谢基因、解毒酶、肠道共生细菌和增效剂等多个方面对[此处原文缺失相关内容]的抗性模式和机制进行了研究。在本研究中，在抑制性消减杂交（SSH）文库中注释了51个物种和149个解毒代谢基因，并在该文库中筛选了12个与高效氯氰菊酯抗性相关的候选基因并进行了定量表达。发现有两个基因在卵期上调，三个基因在幼虫期上调，一个基因在蛹期上调，五个基因在成虫期上调，并且发现有四个基因在中肠和中肠的马氏管中上调。[此处原文缺失相关基因名称]基因的表达在抗性品系中上调，其中在中肠和马氏管中上调最为明显。这些结果为检疫性昆虫抗药性的研究提供了新的见解。
