筋骨草的化学成分及其黑素生成作用和作用机制。

Chemical constituents of Ruta graveolens L. and their melanogenic effects and action mechanism.

机构信息

State Key Laboratory Basis of Xinjiang Indigenous Medicinal Plants Resource Utilization, Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing South Road 40-1, Urumqi 830011, Xinjiang, China; University of Chinese Academy of Sciences, Beijing 100039, China.

出版信息

Fitoterapia. 2022 Jan;156:105094. doi: 10.1016/j.fitote.2021.105094. Epub 2021 Dec 1.

DOI:10.1016/j.fitote.2021.105094

PMID:34861325

Abstract

Ruta graveolens L. has been widely used to treat various skin ailments, especially vitiligo. In this study, we isolated a new furanocoumarin named Rutagrarin (1) along with 14 known compounds (2-15) from the aerial parts of R. graveolens and elucidated their chemical structures via various spectroscopy. We found that compound 5 promoted melanogenesis and tyrosinase activity in B16 cells. Further investigation on underlying mechanisms revealed that compound 5 activated the transcription of microtia-related transcription factors and promoted the production of melanin in B16 cells via the Akt/GSK-3β/β-catenin pathway. Therefore, we confirmed the traditional efficacy of R. graveolens and speculated that compound 5 could be used as a natural drug to treat vitiligo.

摘要

瑞香狼毒已被广泛用于治疗各种皮肤疾病，尤其是白癜风。在本研究中，我们从瑞香狼毒的地上部分分离得到了一个新的呋喃香豆素，命名为 Rutagrarin（1），并结合其他 14 种已知化合物（2-15）阐明了它们的化学结构，通过各种光谱技术。我们发现化合物 5 可促进 B16 细胞中的黑色素生成和酪氨酸酶活性。进一步的机制研究表明，化合物 5 通过 Akt/GSK-3β/β-catenin 通路激活小眼相关转录因子的转录，并促进 B16 细胞中黑色素的生成。因此，我们证实了瑞香狼毒的传统功效，并推测化合物 5 可作为治疗白癜风的天然药物。