Wubuli Ayixiamuguli, Abdulla Rahima, Zang Deng, Jiang Lan, Chen Longyi, Aisa Haji Akber
State Key Laboratory Basis of Xinjiang Indigenous Medicinal Plants Resource Utilization, Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Urumqi 830011, China; University of Chinese Academy of Sciences, Beijing 100049, China.
State Key Laboratory Basis of Xinjiang Indigenous Medicinal Plants Resource Utilization, Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Urumqi 830011, China.
J Chromatogr B Analyt Technol Biomed Life Sci. 2023 Apr 15;1221:123683. doi: 10.1016/j.jchromb.2023.123683. Epub 2023 Mar 20.
A total of 29 batches of R. graveolens were used in this study, their fingerprints were obtained by ultra-performance liquid chromatography (UPLC) and their melanogenesis activities were evaluated. The common peaks were identified by quadrupole-orbitrap high-resolution mass spectrometry (Q-Orbitrap-HRMS). Eleven coumarins, six alkaloids, three flavonoids, three phenolic acids, and four other compounds were found. The spectrum-effect relationships between R. graveolens' chemical fingerprints, the melanin synthesis, and tyrosine's activation activities were established through chemometrics methods which in detail principal component analysis (PCA), gray correlation analysis (GRA), bivariate correlation analysis (BCA) and orthogonal partial least squares analysis (OPLS). The results showed that P18 (bergapten), P22 (isoimperatorin), P15 (kokusaginine), P7 (rutin), P12 (psoralen), and P13 (graveolinine) were relevant to intracellular melanin synthesis activity and tyrosinase activity. Among them, P18 (bergapten), P15 (kokusaginine), and P12 (psoralen) were validated with good melanogenesis activities. This study provides a research basis for future quality control and medicinal application of R. graveolens.
本研究共使用了29批迷迭香，通过超高效液相色谱（UPLC）获得其指纹图谱，并评估其黑素生成活性。通过四极杆-轨道阱高分辨率质谱（Q-Orbitrap-HRMS）鉴定了共有峰。发现了11种香豆素、6种生物碱、3种黄酮类化合物、3种酚酸和4种其他化合物。通过化学计量学方法，具体为主成分分析（PCA）、灰色关联分析（GRA）、双变量相关分析（BCA）和正交偏最小二乘法分析（OPLS），建立了迷迭香化学指纹图谱、黑色素合成及酪氨酸激活活性之间的谱效关系。结果表明，P18（补骨脂素）、P22（异欧前胡素）、P15（黄柏酮碱）、P7（芦丁）、P12（补骨脂内酯）和P13（迷迭香宁碱）与细胞内黑色素合成活性和酪氨酸酶活性相关。其中，P18（补骨脂素）、P15（黄柏酮碱）和P12（补骨脂内酯）被证实具有良好的黑素生成活性。本研究为迷迭香未来的质量控制和药用应用提供了研究依据。
