Key Laboratory of Cold Chain Logistics Technology for Agro-Product, Ministry of Agriculture and Rural Affairs, Institute of Agro-Products Processing and Nuclear Agricultural Technology, Hubei Academy of Agricultural Sciences, Wuhan 430064, China.
Key Laboratory of Fermentation Engineering, Ministry of Education, Hubei Provincial Cooperative Innovation Center of Industrial Fermentation, Hubei Key Laboratory of Industrial Microbiology, Hubei University of Technology, Wuhan 430068, China.
Nutrients. 2023 May 24;15(11):2449. doi: 10.3390/nu15112449.
The mechanism of silver carp scale collagen peptides (SCPs1) on melanogenesis and its mechanism of action were examined in mouse melanoma cells (B16). The cell viability and effects of SCPs1 on intracellular tyrosinase (TYR) activity and melanin, reactive oxygen species (ROS), glutathione (GSH) and cyclic adenosine monophosphate (cAMP) content were examined. The regulatory mechanism of SCPs1 on the cAMP response element-binding protein (CREB) signaling pathway was analyzed. The cell viability of the SCPs1 group was >80% (0.01-1 mg/mL) and the inhibitory rate of SCPs1 on B16 cell melanin increased in a dose-dependent manner. The highest inhibitory rate of SCPs1 on melanin content reaching 80.24%. SCPs1 significantly increased the GSH content and decreased the tyrosinase activity, as well as the content of ROS and cAMP. Western blot analysis showed that SCPs1 significantly inhibited melanocortin-1 receptor (MC1R) expression and CREB phosphorylation in the cAMP-CREB signaling pathway, leading to downregulation of microphthalmia-associated transcription factor (MITF) and the expression of TYR, TYR-related protein-1 (TRP-1) and TRP-2. SCPs1 also inhibited the expression of MC1R, MITF, TYR, TRP-1 and TRP-2 at the transcriptional level. Taken together, SCPs1 inhibited melanin synthesis through the downregulation of the cAMP-CREB signaling pathway. Fish-derived collagen peptides could potentially be applied in skin whitening products.
银鲤鱼鳞片胶原蛋白肽（SCPs1）对黑素生成的作用机制及其作用机制在小鼠黑色素瘤细胞（B16）中进行了研究。检测了细胞活力以及 SCPs1 对细胞内酪氨酸酶（TYR）活性和黑色素、活性氧（ROS）、谷胱甘肽（GSH）和环磷酸腺苷（cAMP）含量的影响。分析了 SCPs1 对环磷酸腺苷反应元件结合蛋白（CREB）信号通路的调节机制。SCPs1 组细胞活力>80%（0.01-1mg/mL），SCPs1 对 B16 细胞黑色素的抑制率呈剂量依赖性增加。SCPs1 对黑色素含量的最高抑制率达到 80.24%。SCPs1 显著增加 GSH 含量，降低酪氨酸酶活性以及 ROS 和 cAMP 的含量。Western blot 分析表明，SCPs1 显著抑制 cAMP-CREB 信号通路中促黑激素-1 受体（MC1R）的表达和 CREB 磷酸化，导致小眼畸形相关转录因子（MITF）和 TYR、TYR 相关蛋白-1（TRP-1）和 TRP-2 的表达下调。SCPs1 还在转录水平抑制 MC1R、MITF、TYR、TRP-1 和 TRP-2 的表达。总之，SCPs1 通过下调 cAMP-CREB 信号通路抑制黑色素合成。鱼类胶原蛋白肽可能在皮肤美白产品中得到应用。
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