Barbul A, Knud-Hansen J, Wasserkrug H L, Efron G
J Surg Res. 1986 Apr;40(4):315-9. doi: 10.1016/0022-4804(86)90193-9.
Antigen-stimulated lymphocytes secrete lymphokines which have been shown to enhance in vitro fibroblast migration, proliferation, and protein synthesis. In the present experiments, the effect of human recombinant interleukin 2 (RIL-2) on wound healing was assessed in vivo. Groups of male Lewis rats, 225-250 g, underwent intraperitoneal insertion of osmotic pumps and a 7-cm dorsal skin incision with subcutaneous placement of polyvinyl alcohol sponges under anesthesia. The dorsal wounds were closed with stainless-steel sutures. The dose of RIL-2 administered was 60,000 u/rat/day for 7 days in experiment I, and 140,000 u/rat/day for 7 days in experiment II. Controls received equal volumes of excipient. Animals were sacrificed 10 days post wounding and wound healing was assessed by fresh breaking strength, fixed breaking strength (following 72 hr of Formalin fixation which maximally crosslinks the collagen present), and sponge hydroxyproline content (an index of reparative collagen accumulation). In vivo RIL-2 administration significantly augmented wound fresh and fixed breaking strength and wound collagen synthesis. Higher doses of RIL-2 (experiment II) did not result in further increases in the parameters studied. The data suggest that lymphocytes participate directly in the process of wound healing.
抗原刺激的淋巴细胞分泌淋巴因子，这些淋巴因子已被证明能在体外增强成纤维细胞的迁移、增殖和蛋白质合成。在本实验中，评估了重组人白细胞介素2（RIL-2）在体内对伤口愈合的作用。将体重225 - 250克的雄性Lewis大鼠分组，在麻醉下进行腹腔内植入渗透泵，并在背部做一个7厘米的皮肤切口，皮下放置聚乙烯醇海绵。背部伤口用不锈钢缝线缝合。在实验I中，给予RIL-2的剂量为60,000单位/大鼠/天，持续7天；在实验II中，剂量为140,000单位/大鼠/天，持续7天。对照组给予等量的赋形剂。在受伤后10天处死动物，通过新鲜断裂强度、固定断裂强度（在福尔马林固定72小时后，此时胶原最大程度交联）和海绵羟脯氨酸含量（修复性胶原积累的指标）来评估伤口愈合情况。在体内给予RIL-2显著提高了伤口的新鲜和固定断裂强度以及伤口胶原合成。更高剂量的RIL-2（实验II）并未导致所研究参数的进一步增加。数据表明淋巴细胞直接参与伤口愈合过程。
