Peterson J M, Barbul A, Breslin R J, Wasserkrug H L, Efron G
Surgery. 1987 Aug;102(2):300-5.
To determine the importance of T-lymphocytes in wound healing, we examined the effect of T-lymphocyte depletion on the healing of surgical wounds. Thirty Balb/c mice were injected intraperitoneally with 1 mg of rat anti-mouse (IgG2b) cytotoxic monoclonal antibody (30H12) against the Thy1.2 (all T) determinant. Twenty-four hours later animals showed a greater than 95% depletion of Thy1.2 cells in peripheral blood and spleen. Thirty control mice received nonspecific rat immunoglobulin (1 mg). Twenty-four hours after treatment mice underwent a 2.5 cm dorsal skin incision with subcutaneous placement of polyvinyl alcohol sponges. Injections were repeated at weekly intervals. Wound healing was assessed at 2, 3, and 4 weeks by the breaking strength of wound strips and by the hydroxyproline content of sponge granulomas (an index of wound reparative collagen deposition). Thy1.2 depletion at death was 95% to 57% in peripheral blood and 86% to 68% in the spleen. Both groups gained weight equally. We found that T cell depletion significantly impairs wound breaking strength and wound collagen deposition at all times studied. The data strongly suggest that T-lymphocytes modulate fibroblast activity during normal wound healing.
为了确定T淋巴细胞在伤口愈合中的重要性，我们研究了T淋巴细胞耗竭对手术伤口愈合的影响。30只Balb/c小鼠腹腔注射1mg抗Thy1.2（所有T细胞）决定簇的大鼠抗小鼠（IgG2b）细胞毒性单克隆抗体（30H12）。24小时后，动物外周血和脾脏中Thy1.2细胞的耗竭率超过95%。30只对照小鼠接受非特异性大鼠免疫球蛋白（1mg）。处理24小时后，小鼠进行2.5cm背部皮肤切口，并皮下植入聚乙烯醇海绵。每周重复注射。在第2、3和4周通过伤口条带的断裂强度和海绵肉芽肿的羟脯氨酸含量（伤口修复性胶原沉积的指标）评估伤口愈合情况。死亡时外周血中Thy1.2细胞的耗竭率为95%至57%，脾脏中为86%至68%。两组体重增加相同。我们发现，在所有研究时间点，T细胞耗竭均显著损害伤口断裂强度和伤口胶原沉积。数据强烈表明，在正常伤口愈合过程中，T淋巴细胞调节成纤维细胞活性。
