Zhang Ying, Liu Zhanzhi, Li Guangyao, Fu Xueni, Zhang Yucheng, Wang Zhiyuan, Tian Yaping, Wu Jing
State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, Jiangsu, China.
Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Bioengineering, Jiangnan University, Wuxi 214122, Jiangsu, China.
Sheng Wu Gong Cheng Xue Bao. 2022 Jan 25;38(1):217-225. doi: 10.13345/j.cjb.210036.
With the development of global economy, the dramatically increased production of polyethylene terephthalate (PET) plastics has led to a remarkably increased amount of plastic waste. PET waste can be treated by landfill, incineration, or biodegradation. While landfilling and incineration may cause secondary pollution, biodegradation has since received increased attentions due to its environmental friendliness. Recent studies have indicated that the carbohydrate binding module (CBM) can effectively enhance the binding of PET degrading enzymes to PET, and consequently increasing PET degradation rate. Here we constructed a fusion protein CBM2-Tfuc containing the CBM2 from and the cutinase Tfuc from , by megaprimer PCR of whole plasmids (MEGAWHOP). Notabaly, the PET film degradation efficiency (at 60 ℃) of CBM2-Tfuc was 2.8 times that of Tfuc. This study may provide technical support for constructing fusion proteins capable of efficiently degrading PET.
随着全球经济的发展，聚对苯二甲酸乙二酯（PET）塑料产量的急剧增加导致了塑料废弃物数量的显著增长。PET废弃物可通过填埋、焚烧或生物降解进行处理。虽然填埋和焚烧可能会造成二次污染，但生物降解因其环境友好性而受到越来越多的关注。最近的研究表明，碳水化合物结合模块（CBM）可以有效增强PET降解酶与PET的结合，从而提高PET降解率。在此，我们通过全质粒大引物PCR（MEGAWHOP）构建了一种融合蛋白CBM2-Tfuc，其包含来自[具体来源1]的CBM2和来自[具体来源2]的角质酶Tfuc。值得注意的是，CBM2-Tfuc在60℃时对PET薄膜的降解效率是Tfuc的2.8倍。本研究可为构建能够高效降解PET的融合蛋白提供技术支持。
