葡萄糖酸奥替尼啶对诺如病毒的消毒效果及作用机制。

Disinfection efficacy and mechanism of olanexidine gluconate against norovirus.

机构信息

Naruto Research Institute, Research and Development Center, Otsuka Pharmaceutical Factory, Inc., Naruto, Tokushima, Japan; Department of Frontier Sciences for Advanced Environment, Graduate School of Environmental Studies, Tohoku University, Sendai, Miyagi, Japan.

Naruto Research Institute, Research and Development Center, Otsuka Pharmaceutical Factory, Inc., Naruto, Tokushima, Japan.

出版信息

Am J Infect Control. 2022 Jul;50(7):764-771. doi: 10.1016/j.ajic.2021.11.020. Epub 2021 Dec 2.

DOI:10.1016/j.ajic.2021.11.020

PMID:34864086

Abstract

BACKGROUND

The purpose of this study was to evaluate the virucidal activity of a new olanexidine-containing formulation for hand hygiene (olanexidine gluconate hand rub; OLG-HR) against non-enveloped viruses and to understand its mechanism of action.

METHODS

The virucidal activities of OLG-HR against two strains of caliciviruses and three adenovirus serotypes were evaluated through suspension tests. Also, virus-like particles were used to predict the effect of olanexidine gluconate on virus particle structure.

RESULTS

The results of suspension tests under conditions with and without interfering substances (1.5% BSA) indicated that OLG-HR had a broad-spectrum effect against non-enveloped viruses, and the virucidal effect was unaffected by organic contaminants. Furthermore, olanexidine inhibited the binding ability of virus-like particles to the binding receptor of human norovirus and increased the aggregation of virus-like particles in a dose-dependent manner. Transmission electron microscopy showed that the morphology of the virus-like particles was affected by exposure to olanexidine, indicating that the protein-denaturing effect of olanexidine gluconate caused the loss of receptor-binding capability of the viral capsid protein.

CONCLUSIONS

This study suggests that olanexidine gluconate is a potential biological and environmental disinfectant against norovirus and adenovirus.

摘要

背景

本研究旨在评估一种新型含奥拉西啶的手卫生制剂（奥拉西啶葡萄糖酸盐手消毒剂；OLG-HR）对非包膜病毒的病毒杀灭活性，并了解其作用机制。

方法

通过悬浮试验评估 OLG-HR 对两种肠病毒株和三种腺病毒血清型的病毒杀灭活性。此外，还使用病毒样颗粒来预测奥拉西啶葡萄糖酸盐对病毒颗粒结构的影响。

结果

在有和没有干扰物质（1.5%BSA）的悬浮试验条件下，结果表明 OLG-HR 对非包膜病毒具有广谱作用，且病毒杀灭效果不受有机污染物的影响。此外，奥拉西啶抑制了病毒样颗粒与人诺如病毒结合受体的结合能力，并呈剂量依赖性增加病毒样颗粒的聚集。透射电子显微镜显示，病毒样颗粒的形态受奥拉西啶暴露的影响，表明奥拉西啶葡萄糖酸盐的蛋白变性作用导致病毒衣壳蛋白丧失受体结合能力。